Recombinant Mouse Semaphorin 4A mFc Chimera Protein, CF

Catalog #: 11096-S4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11096-S4-050

Recombinant Mouse Semaphorin 4A mFc Chimera Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Semaphorin 4A mFc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Semaphorin 4A mFc Chimera (Catalog # 11096-S4) is immobilized at 2.0 µg/mL (100 µL/well), the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein (Catalog # 2078-T4) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse Semaphorin 4A protein

Mouse Sema 4A (Thr33-Phe683) Accession # Q62178.2	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q62178.2

N-terminal Sequence
Analysis

Thr33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

99 kDa

SDS-PAGE

90-110 kDa, under reducing conditions.

Product Datasheets

11096-S4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11096-S4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse Semaphorin 4A mFc Chimera Protein (Catalog #11096-S4) is immobilized at 2.0 µg/mL (100 µL/well), the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Protein (2078-T4) that produces 50% of the optimal binding response is 0.100-0.600 μg/mL

SDS-PAGE

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2 μg/lane of Recombinant Mouse Semaphorin 4A mFc Chimera Protein (Catalog # 11096-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-110 kDa and 180-220 kDa, respectively.

Reconstitution Calculator

Background: Semaphorin 4A

Semaphorin 4A (Sema4A), also known as Semaphorin-B (SEMB), is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems (1). Semaphorins are a large family of secreted and membrane-bound glycoproteins, divided into eight subclasses, that were initially implicated in axon guidance and neural development. Mature Sema4a consists of an extracellular domain (ECD) containing sema, PSI and C2-type immunoglobulin domains, a transmembrane domain, and a cytoplasmic domain with two Ser/Thr phosphorylation sites. The ECD of mature mouse Sema4A shares 87% and 94% amino acid sequence identity with human and rat Sema4a, respectively. Several splice variants have been reported with varying truncations in the ECD, transmembrane or cytoplasmic domains (3). Sema4A was first described as a molecule that enhances T cell activation and interacts with TIM-2 (T cell immunoglobulin and mucin domain-2 (4). Sema4a has also been shown to bind to the receptor ILT-4 on activated CD4+ cells (5). Mice with targeted disruption of Sema4A show defects in dendritic cell-mediated T cell priming and Th1 responses (6). Roles for Sema4A have also been identified in the brain, the endothelium and the eye. It mediates hippocampal neuron growth cone collapse in vitro through interaction of the sema domain with plexin-B1 (7). Interaction of Sema4A with endothelial cell plexin-D1 causes opposition to the angiogenic, proliferative, chemotactic and integrin-mediated adhesive actions of VEGF (8). The retina of Sema4A-/- mice shows severe degeneration, and mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans (9, 10).

References

Kumanogoh, A. et al. (2003) J. Cell Sci. 116:3463.
Swissprot Accession # Q9H3S1.
Entrez Accession # CAI15528, CAI15529, CAI15531, CAI15532, CAI15533 and EAW52993.
Kumanogoh, A. et al. (2002) Nature 419:629.
Lu, N. et al. (2018) Nature Commun. 9:742.
Kumanogoh, A. et al. (2005) Immunity 22:305.
Yukawa, K. et al. (2005) Int. J. Mol. Med. 16:115.
Toyofuku, T. et al. (2007) EMBO J. 26:1373.
Rice, D.S. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:2767.
Abid, A. et al. (2007) J. Med. Genet. 43:378.

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Alternate Names

CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287