Recombinant Mouse Semaphorin 4C Fc Chimera Protein, CF

Catalog #: 8394-S4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8394-S4-050

Recombinant Mouse Semaphorin 4C Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Semaphorin 4C Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit survival of SK‑OV‑3 human ovarian carcinoma cells. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Semaphorin 4C protein

Mouse Semaphorin 4C (Ala21-Gly664) Accession # Q64151	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q64151

N-terminal Sequence
Analysis

Ala21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

99 kDa

SDS-PAGE

105-125 kDa, reducing conditions

Product Datasheets

8394-S4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8394-S4

Formulation	Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl, pH 5.0
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Semaphorin 4C (Catalog # 8394-S4) inhibits survival of SK‑OV‑3 human ovarian carcinoma cells. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Reconstitution Calculator

Background: Semaphorin 4C

Semaphorin 4C (Sema4C; also Sema I and M-SemaF) is a member of the class IV semaphorin family of type 1 transmembrane glycoproteins (1). Semaphorin 4C signaling is involved in cell attraction and repulsion as well as neural tube closure, stem cell proliferation, and kidney development. Mature Semaphorin 4C consists of a 642 amino acid (aa) extracellular region, a 20 aa transmembrane domain, and a 148 aa PDZ-containing cytoplasmic tail (2-4). The extracellular domain of mature mouse Semaphorin 4C shares 85% and 94% aa sequence identity with human and rat Semaphorin 4C, respectively, and is characterized by a Sema domain, a cysteine rich PSI domain, and an Ig-like C2-type domain. Semaphorin 4C is widely expressed during embryogenesis with high expression within the neural tube (5-7). Comparison of Plexin B2^-/- and Sema4C^-/- mice revealed similar phenotypes, including neural tube closure defects and neonatal lethality. Viable Sema4C^-/- neonates have an abnormally developed cerebellum and defects in ventral skin pigmentation (8). Semaphorin 4C has been shown to induce cerebellar granule cell precursor migration and neural stem cell proliferation in in vitro and ex vivo assays, respectively (9). Involvement of Semaphorin 4C in neural stem cell proliferation is further supported by the down-regulation of its expression in neuroblasts during differentiation and up-regulation in proliferating Nestin-postive cells following ischemia (2). Semaphorin 4C is a high affinity ligand for Plexin B2. Upon activation by Sema4C, Plexin B2 can phosphorylate Tyr 1248 on the receptor tyrosine kinase, ErbB2, which then activates RhoA to regulate cytoskeletal dynamics as well as neuronal migration and proliferation (9, 10). During embryogenesis, Semaphorin 4C-Plexin B2 signaling also stimulates branching of the ureteric epithelium in the kidney (11). In adult tissues, the expression pattern of Semaphorin 4C in non-neuronal tissues indicates that Semaphorin 4C-Plexin B2 signaling may additionally regulate vascular and endocrine function (12). Similar to Semaphorin 3A (13, 14), R&D Systems’ in‑house testing data indicate that Semaphorin 4C can inhibit cell survival.

References

Tessier-Lavigne, M. and C.S. Goodman (1996) Science 274:1123.
Wu, H. et al. (2009) J. Mol. Neurosci. 39:27.
Wang, L. et al. (1999) J. Biol. Chem. 274:14137.
Inagaki, S. et al. (1995) FEBS Lett. 370:269.
Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
Perala, N. et al. (2010) Dev. Dyn. 239:2722.
Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
Maier, V. et al. (2011) Mol. Cell. Neurosci. 46:419.
Deng, S. et al. (2007) J. Neurosci. 27:6333.
Swiercz, J.M. et al. (2004) J. Cell. Biol. 165:869.
Perala, N. et al. (2011) Differentiation 81:81.
Zielonka, M. et al. (2010) Exp. Cell Res. 316:2477.
Shirvan, A. et al. (1999) J. Neurochem. 73:961.
Guttman-Raviv, N. et al. (2007) J. Biol. Chem. 282:26294.

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Alternate Names

FLJ20369; KIAA1739; MGC126382; MGC126383; M-Sema F; M-SEMA-F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; Semaf; SEMAI; Semaphorin 4C; semaphorin-4C