Recombinant Mouse Semaphorin 4F Fc Chimera Protein, CF
Recombinant Mouse Semaphorin 4F Fc Chimera Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
| Mouse Semaphorin 4F (Arg41-Gly667) Accession # Q9Z123 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
7200-S4
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Semaphorin 4F
Semaphorin 4F (Sema4F; previously called Sema W) is a 100-150 kDa member of the class 4 family of type I transmembrane semaphorins (1-3). Mature mouse Sema4F is a 737 amino acid (aa) protein that contains a Sema domain, a PSI region, and a C2-type Ig-like domain within its extracellular domain (ECD; aa 41-667) (2). Within the ECD, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively. Sema4F is expressed by cell types that include Schwann cells, oligodendrocyte (highest in precursors), retinal ganglion cells, and neurons, especially along migratory pathways (2-4). It is found within post-synaptic densities at glutamatergic synapses, where it binds and probably recruits the cytoplasmic scaffolding protein PSD-95 (3, 5). In Schwann cells, Sema4F likely acts as an adhesive protein and a mediator of contact inhibition, and thus an inhibitor of cell proliferation (6). In neurofibromatosis type 1 (NT1), loss of the NF1 protein results in loss of Schwann cell Sema4F expression via perturbation of ERK signals (6). Loss of Sema4F in turn disrupts adhesive interaction and alignment of Schwann cells with neurons (6). For oligodendroglial precursors in culture, immobilized Sema4F inhibits migration and enhances survival, differentiation and adhesion (3). A secreted form of approximately 80 kDa is reported in prostate cancer cells, transfected cell culture supernatants, and adult brain lysates (3, 7).
- Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
- Encinas, J.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2491.
- Armendariz, B.G. et al. (2012) Mol. Cell. Neurosci. 49:54.
- Cohen, R.I. et al. (2003) J. Neurochem. 85:1262.
- Schultze, W. et al. (2001) J. Neurochem. 78:482.
- Parrinello, S. et al. (2008) Genes Dev. 22:3335.
- Ayala, G.E. et al. (2008) Clin. Cancer Res. 14:7593.
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