Recombinant Mouse Semaphorin 5A Fc Chimera Protein, CF

Catalog #: 6584-S5 Datasheet / COA / SDS

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6584-S5-025

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Recombinant Mouse Semaphorin 5A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells. The ED₅₀for this effect is 0.9-3.6 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Semaphorin 5A protein

Mouse Semaphorin 5A (Met1 - Thr765) Accession # NP_033180	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

NP_033180

N-terminal Sequence
Analysis

Glu23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

110.8 kDa (monomer)

SDS-PAGE

120-140 kDa, reducing conditions

Product Datasheets

6584-S5

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6584-S5

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Semaphorin 5A

Semaphorin 5A (Sema5A, previously called SemaF) is a 140 kDa protein of the semaphorin family of axon guidance molecules (1 ‑ 4). Class 5 semaphorins are type I transmembrane glycoproteins with an N-terminal Sema domain and multiple juxtamembrane type 1 thrombospondin (TSP) repeats within their extracellular domains (1 ‑ 3). Sema5A is expressed developmentally in oligodendrocytes, neuroepithelial cells surrounding retinal axons, the base of limb buds, the cardiac atrial septum and endocardial cushions, and the mesoderm surrounding cranial vessels (3 ‑ 6). The mouse Sema5A cDNA encodes a 22 amino acid (aa) signal sequence, a 946 aa extracellular domain (ECD), a 24 aa transmembrane sequence and an 85 aa cytoplasmic portion. Within aa 23 ‑ 765, which includes the sema domain and four of the seven TSP repeats, mouse Sema5A shares 93%, 98%, 93%, 92% and 91% aa identity with corresponding human, rat, porcine, bovine and canine sequences. Semaphorins typically transduce signals through transmembrane plexins (1, 2). The sema domain of Sema5A binds plexin B3, triggering signaling via HGF R/c-Met (7). Both Sema5A and plexin B3 are expressed postnatally during differentiation and migration of central nervous system oligodendrocytes. However, plexin B3 is not significantly expressed prenatally and therefore unlikely to be the Sema5A receptor during development (7, 8). The Sema5A TSP repeats interact with either heparin sulfate or chondroitin sulfate proteoglycans (HSPG, CSPG) (9). HSPG interaction promotes attraction, while CSPG interaction promotes repulsion and is essential for axon fasciculation, independent of plexin B3 (9, 10). Sema5A mutations have been implicated in the human genetic syndrome, cri-du-chat, while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson’s (11, 12). Sema5A expression may be up‑regulated in metastatic cancer cells and down‑regulated in autism (13, 14). High expression of Sema5A has been associated with tumor growth and metastasis in pancreatic cancer (15).

References

Flannery, E. and M. Duman-Scheel (2009) Curr. Drug Targets 10:611.
Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
Adams, R.H. et al. (1996) Mech. Dev. 57:33.
Oster, S.F. et al. (2003) Development 130:775.
Goldberg, J.L. et al. (2004) J. Neurosci. 24:4989.
Fiore, R. et al. (2005) Mol. Cell. Biol. 25:2310.
Artigiani, S. et al. (2004) EMBO Rep. 5:710.
Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
Kantor, D.B. et al. (2004) Neuron 44:961.
Hilario, J.D. et al. (2008) Dev. Biol. 326:190.
Simmons, A.D. et al. (1998) Biochem. Biophys. Res. Commun. 242:685.
Lin, L. et al. (2009) Trends Neurosci. 32:142.
Pan, G-Q. et al. (2009) World J. Gastroenterol. 15:2800.
Melin, M. et al. (2006) Neuropsychobiology 54:64.
Sadanandam A. et al. (2010) Int J. Cancer 127(6)1373.

Entrez Gene IDs

9037 (Human); 20356 (Mouse); 310207 (Rat)

Alternate Names

FLJ12815; sema domain, seven thrombospondin repeats (type 1 and type 1-like); Sema F; SEMA5A; SEMAF; Semaphorin 5A; semaphorin F; semaphorin-5A; Semaphorin-F; semF; transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A