Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

Catalog #: 2264-S6 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2264-S6-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF (Catalog # 256-GF).

Source

Human embryonic kidney cell, HEK293-derived mouse Semaphorin 6B protein

Mouse Semaphorin 6B (Leu27-Ser605) Accession # O54951	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

O54951

N-terminal Sequence
Analysis

Leu27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

91 kDa (monomer)

SDS-PAGE

100-115 kDa, reducing conditions

Product Datasheets

2264-S6

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2264-S6

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Semaphorin 6B

Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Mouse Semaphorin 6B has a molecular weight of approximately 120 kDa and is thought to form disulfide-linked homodimers (4). It shares 95% and 99% amino acid (aa) sequence identity with the human and rat orthologs, respectively. In mice, Semaphorin 6B is highly expressed in developing muscle and in subregions of the nervous system, as well as at low levels in most adult tissues (4). It has been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (5, 6). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (7-9).

References

Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
Neufeld, G. et al. (2005) Front. Biosci. 10:751.
Roney, K. et al. (2013) Protein Cell 4:17.
Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
Suto, F. et al. (2005) J. Neurosci. 25:3628.
Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
Correa, R.G. et al. (2001) Genomics 73:343.
Murad, H. et al. (2006) Int. J. Oncol. 28:977.
Ge, C. et al. (2013) J. Int. Med. Res. 41:284.

Entrez Gene IDs

10501 (Human); 20359 (Mouse)

Alternate Names

sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353