Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF
Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF Summary
Product Specifications
Val28-Lys491, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1239-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB
- Recombinant Mouse Serpin F2/ alpha 2‑Antiplasmin (rmSerpin F2) (Catalog # 1239-PI)
- Trypsin (Sigma, Catalog # T-1426)
- Substrate: Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute Trypsin to 0.25 µg/mL in Assay Buffer and KEEP ON ICE.
- Prepare a curve of rmSerpin F2 (MW: 53,503 Da) in Assay Buffer. Make the following serial dilutions: 100, 20, 15, 10, 7, 4, 2, and 1 nM.
- Combine equal volumes of diluted Trypsin and rmSerpin F2 curve dilutions. Include two blank controls containing equal volumes of Assay Buffer and diluted Trypsin without any rmSerpin F2.
- Incubate mixtures at 37 °C for 15 minutes.
- Dilute the reaction mixtures 1/5 with Assay Buffer.
- Dilute Substrate to 20 µM with Assay Buffer.
- Load in a black well plate 50 µL of the incubated mixtures, and start the reaction by adding 50 µL of 20 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in kinetic mode for 5 minutes.
- Derive the 50% inhibiting concentration (IC50) of rmSerping F2 by plotting RFU/min (or specific activity) vs. concentration with 4-PL fitting.
- The specific activity for Trypsin at each point may be determined using the following formula (if needed):
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
- Trypsin: 0.00125 µg
- rmSerpin F2 curve: 5, 1, 0.75, 0.5, 0.35, 0.2, 0.1, and 0.05 nM
- Substrate: 10 µM
Reconstitution Calculator
Background: Serpin F2/alpha 2-Antiplasmin
Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28 to 39) and a mature chain (residues 40 to 491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).
- Menoud, P.-A. et al. (1996) J. Clin. Invest. 97:2478.
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Potempa, J. et al. (1988) Science. 241:699.
- Sumi, Y. et al. (1989) J. Biochem. 106:703.
Citation for Recombinant Mouse Serpin F2/alpha 2-Antiplasmin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Antiplasmin, but not amiloride, prevents synovitis and cartilage damage following hemarthrosis in hemophilic mice.
Authors: Nieuwenhuizen L, Roosendaal G, Mastbergen S, Coeleveld K, Biesma D, Lafeber F, Schutgens R
J Thromb Haemost, 2014-02-01;12(2):237-45.
Species: Mouse
Sample Types: Whole Organism
Applications: In Vivo
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