Recombinant Mouse Siglec-15 Fc Chimera Protein, CF

Catalog #: 10101-SL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10101-SL-050

Recombinant Mouse Siglec-15 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Siglec-15 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 1-6 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Siglec-15 protein

Mouse Siglec-15 (Arg24-Tyr250) Accession # NP_001094508.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_001094508.1

N-terminal Sequence
Analysis

Arg24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

52 kDa

SDS-PAGE

57-64 kDa

Product Datasheets

10101-SL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10101-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Siglec-15 Fc Chimera (Catalog # 10101-SL) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED₅₀ for this effect is 1-6 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Siglec-15 Fc Chimera (Catalog # 10101-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-64 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature mouse Siglec-15 consists of a 239 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse Siglec-15 shares 85% and 92% aa sequence identity with human and rat Siglec-15, respectively. In human Siglec-15, alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (7). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (7).

References

Humphrey, M.B. and M.C. Nakamura (2016) Clin. Rev. Allergy Immunol. 51:48.
Angata, T. et al. (2007) Glycobiology 17:838.
Takamiya, R. et al. (2013) Glycobiology 23:178.
Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
Wang, J et al. (2019) Nat. med. 25:656.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Alternate Names

CD33 antigen-like 3SIGLEC-15; CD33 molecule-like 3; CD33L3; HsT1361; sialic acid binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; Siglec15; Siglec-15