Recombinant Mouse Slit3 Protein, CF
Recombinant Mouse Slit3 Protein, CF Summary
Product Specifications
Ser27-His901, with a C-terminal 10-His tag
Accession # Q9WVB4
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3629-SL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Slit3
Slit3 is a member of the slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1). Like other mammalian family members, the 1523 amino acid (aa), 200 kDa Slit3 contains a signal sequence followed by 23 leucine-rich repeats (LRR) and nine EGF‑like sequences (1). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7, and a C-terminal cysteine-rich domain (1). In Drosophila Slit, specific LRR are sites of ROBO interaction and homodimerization (2). Drosophila Slit shows equal similarities with all three mouse slit proteins, which are 59-66% identical with each other (1). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in function (3). Slit3 is also expressed in the lung, kidney, skeletal muscle and heart, both during development and postnatally (1, 4-6). ROBO2 is often found in complementary areas (4). Mice with genetically disrupted Slit3 have thin diaphragms with disorganized collagen fibrils and frequently develop diaphragmatic hernias (5, 6). Abnormalities in kidney development may also occur (5). Although Slit proteins are generally considered to be secreted, significant amounts of Slit3 may be retained in the mitochondria because of features in the signal sequence that indicate a high probability of mitochondrial targeting (7). Secreted Slit is often membrane-associated (7). Mouse Slit3 shows 98% and 94% amino acid identity with rat and human Slit3, respectively.
- Yuan, W. et al. (1999) Dev. Biol. 212:290.
- Howitt, J. A. et al. (2004) EMBO J. 23:4406.
- Long, H. et al. (2004) Neuron 42:213.
- Greenberg, J. M. et al. (2004) Dev. Dyn. 230:350.
- Liu, J. et al. (2003) Mech. Dev. 120:1059.
- Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
- Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.
Citations for Recombinant Mouse Slit3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis
Authors: W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang
Scientific Reports, 2022-03-14;12(1):4352.
Species: Mouse, N/A
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, ELISA -
Estrogen receptor (ER) agonists differentially regulate neuroangiogenesis in peritoneal endometriosis via the repellent factor SLIT3.
Authors: Greaves E, Collins F, Esnal-Zufiaurre A, Giakoumelou S, Horne A, Saunders P
Endocrinology, 2014-07-22;155(10):4015-26.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital diaphragmatic hernia.
Authors: Zhang, Bing, Xiao, Wenyuan, Qiu, Hong, Zhang, Fuming, Moniz, Heather, Jaworski, Alexande, Condac, Eduard, Gutierrez-Sanchez, Gerardo, Heiss, Christia, Clugston, Robin D, Azadi, Parastoo, Greer, John J, Bergmann, Carl, Moremen, Kelley W, Li, Dean, Linhardt, Robert J, Esko, Jeffrey, Wang, Lianchun
J Clin Invest, 2013-12-20;124(1):209-21.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The chemorepellent Slit3 promotes monocyte migration.
Authors: Geutskens SB, Hordijk PL, van Hennik PB
J. Immunol., 2010-11-15;185(12):7691-8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse.
Authors: Naska S, Lin DC, Miller FD
Mol. Cell. Neurosci., 2010-06-17;45(2):108-20.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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