Recombinant Mouse SLITRK1 Protein, CF Summary
Product Specifications
Asn18-Ser616, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9460-SK
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: SLITRK1
SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and a Trk-like cytoplasmic region except SLITRK1 (3). Mouse SLITRK1 is a type I transmembrane protein of 696 amino acids (aa). It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in Slit. Each LRD contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR. The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD, their significance is unclear (5). One truncating frameshift mutation (a unique 27 aa substitution after Arg421) is associated with Tourette's Syndrome (6). As noted, the cytoplasmic region of SLITRK1 is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2-6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2-6 block neurite extension (3) and bind and form complexes with LAR-RPTP's at functional synapses (7). Mouse SLITRK1 shares 98% and 94% aa sequence identity with human and rat SLITRK1, respectively.
- Aruga, J. et al. (2003) Gene 315:87.
- Nagase, T. et al. (2001) DNA Res. 8:179.
- Aruga, J. & K. Mikoshiba, (2003) Mol. Cell. Neurosci. 24:117.
- Enkhbayar, P. et al. (2003) Proteins 54:394.
- Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
- Abelson, J.F. et al. (2005) Science 310:317.
- Um, J.W. et al. (2014) Nat. Commun. 5:5423.
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