Recombinant Mouse SLITRK3 His-tag Protein, CF

Catalog #: 1639-SK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1639-SK-050

Recombinant Mouse SLITRK3 His-tag Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SLITRK3 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng of protein on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived mouse SLITRK3 protein
Thr30-Ser655, with a C-terminal 6-His tag

Accession #

Q810B9

N-terminal Sequence
Analysis

Thr30

Predicted Molecular Mass

72 kDa

SDS-PAGE

94-105 kDa, reducing conditions

Product Datasheets

1639-SK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1639-SK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Mouse SLITRK3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 94-105 kDa.

Reconstitution Calculator

Background: SLITRK3

SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK3 contains 6 leucine rich repeats (LRR), a C‑terminal LRR domain, and 6 more LRR flanked by a pair of N- and C‑terminal LRR domains (2, 3). Within the ECD, mouse SLITRK3 shares 98% and 99.5% amino acid sequence identity with human and rat SLITRK3, respectively. SLITRK3 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-6). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is up-regulated during the development of gastrointestinal stromal tumors (7).

References