Recombinant Mouse SMOC-1 Protein, CF

Catalog #: 5550-SM
1 Citation Datasheet / COA / SDS

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5550-SM-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SMOC-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

When 5 x 10⁴ cells/well are added to rmSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED₅₀ for this effect is 1-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse SMOC-1 protein
His26-Val452, with a C-terminal 6-His tag

Accession #

AAD41590

N-terminal Sequence
Analysis

His26 & Ile35

Predicted Molecular Mass

47.9 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

5550-SM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5550-SM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SMOC-1

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70 - 90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules (1). Mature mouse SMOC-1 is 427 amino acids (aa) in length. It contains one Kazal-like domain (aa 42 - 86), two thyroglobulin type-1 segments (aa 91 - 157 and223 - 291) and two functional EF-hand sequences (aa 358 - 393 and 395 - 430) (1, 2). Two splice variants contain an insertion of eleven aa after Lys174, with one of these also including an alternate start site at Met65 (3). Mature mouse SMOC-1 shares 99% aa identity with rat SMOC-1 and 92% aa identity with human, canine and bovine SMOC-1. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida (1). It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers (4). Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin (2). A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta -regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta, for example in the glomerulus (5). In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

References

Vannahme, C. et al. (2002) J. Biol. Chem. 277:37977.
Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
Entrez Q8BLY1 and EDL02699.
Gersdorff, N. et al. (2006) Histochem. Cell Biol. 126:705.
Dreieicher, E. et al. (2009) J. Am. Soc. Nephrol. 20:1963.
Thomas, J.T. et al. (2009) J. Biol. Chem. 284:18994.

Long Name

Secreted Modular Calcium-binding Protein 1

Entrez Gene IDs

64093 (Human); 64075 (Mouse); 314280 (Rat)

Alternate Names

Secreted modular calcium-binding protein 1; SMOC1; SMOC-1; SPARC related modular calcium binding 1; SPARC-related modular calcium-binding protein 1

Related Research Areas

Citation for Recombinant Mouse SMOC-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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