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Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus

Catalog #: 464-SH
69 Citations 2 Reviews Datasheet / COA / SDS
Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
464-SH-025/CF
464-SH-200/CF

With Carrier

Catalog # Availability Size / Price Qty
464-SH-025
464-SH-200
464-SH-01M
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Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus SEC-MALS
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Product Details
Citations (69)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.05-0.25 µg/mL.
Source
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198 (Cys25Ile-Ile), with an N-terminal Met
Accession #
Q62226
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
19.8 kDa

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464-SH (with carrier)

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COA
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464-SH/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

464-SH

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

464-SH/CF

Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and DTT.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus Bioactivity View Larger

Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.

SDS-PAGE Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.

Cell Culture Staining of dopaminergic neurons with anti-tyrosine hydroxylase in green, anti-neuron-specific beta III tubulin Tuj1 in red and DAPI in blue View Larger

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (Catalog # 464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue).

Cell Culture Staining of dopaminergic neurons with anti-tyrosine hydroxylase in red, anti-neuron-specific beta III tubulin Tuj1 in green and DAPI in blue View Larger

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (Catalog # 464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green). Cells were counterstained with DAPI (5748; blue).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).

References
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

Citations for Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

69 Citations: Showing 1 - 10
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  1. Brain organoid maturation and implantation integration based on electrical signals input
    Authors: Li, XH;Hu, N;Chang, ZH;Shi, JX;Fan, X;Chen, MM;Bao, SQ;Chen, C;Zuo, JC;Zhang, XW;Wang, JJ;Ming, D;
    Journal of advanced research
    Species: Human
    Sample Types: Embryo
    Applications: Bioassay
  2. Huntington's disease cellular phenotypes are rescued non-cell autonomously by healthy cells in mosaic telencephalic organoids
    Authors: Galimberti, M;Nucera, MR;Bocchi, VD;Conforti, P;Vezzoli, E;Cereda, M;Maffezzini, C;Iennaco, R;Scolz, A;Falqui, A;Cordiglieri, C;Cremona, M;Espuny-Camacho, I;Faedo, A;Felsenfeld, DP;Vogt, TF;Ranzani, V;Zuccato, C;Besusso, D;Cattaneo, E;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration
    Authors: Saurat, N;Minotti, AP;Rahman, MT;Sikder, T;Zhang, C;Cornacchia, D;Jungverdorben, J;Ciceri, G;Betel, D;Studer, L;
    Cell stem cell
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Parkinsonism Sac domain mutation in Synaptojanin-1 affects ciliary properties in iPSC-derived dopaminergic neurons
    Authors: Mohd Rafiq, N;Fujise, K;Rosenfeld, MS;Xu, P;De Camilli, P;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Multiple factors to assist human-derived induced pluripotent stem cells to efficiently differentiate into midbrain dopaminergic neurons
    Authors: Chen, Y;Kuang, J;Niu, Y;Zhu, H;Chen, X;So, KF;Xu, A;Shi, L;
    Neural regeneration research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease
    Authors: Busquets, O;Li, H;Syed, KM;Jerez, PA;Dunnack, J;Bu, RL;Verma, Y;Pangilinan, GR;Martin, A;Straub, J;Du, Y;Simon, VM;Poser, S;Bush, Z;Diaz, J;Sahagun, A;Gao, J;Hernandez, DG;Levine, KS;Booth, EO;Bateup, HS;Rio, DC;Hockemeyer, D;Blauwendraat, C;Soldner, F;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development
    Authors: F Hermans, L Hemeryck, C Bueds, M Torres Per, S Hasevoets, H Kobayashi, D Lambrechts, I Lambrichts, A Bronckaers, H Vankelecom
    Stem Cell Reports, 2023-04-20;18(5):1166-1181.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  8. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023-03-02;30(3):264-282.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Parallel Neurodegenerative Phenotypes in Sporadic Parkinson's Disease Fibroblasts and Midbrain Dopamine Neurons
    Authors: MJ Corenblum, A McRobbie-J, E Carruth, K Bernard, M Luo, LJ Mandarino, S Peterson, D Billheimer, T Maley, ED Eggers, L Madhavan
    bioRxiv : the preprint server for biology, 2023-02-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation
    Authors: PR Wu, SY Chiang, R Midence, WC Kao, CL Lai, IC Cheng, SJ Chou, CC Chen, CY Huang, RH Chen
    Cell Death & Disease, 2023-01-21;14(1):52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers
    Authors: S Wu, NC Hernandez, DW Sirkis, I Thomas-Wri, R Wade-Marti, R Schekman
    Elife, 2023-01-10;12(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. hPSC-derived sacral neural crest enables rescue in a severe model of Hirschsprung's disease
    Authors: Y Fan, J Hackland, A Baggiolini, LY Hung, H Zhao, P Zumbo, P Oberst, AP Minotti, E Hergenrede, S Najjar, Z Huang, NM Cruz, A Zhong, M Sidharta, T Zhou, E de Stanchi, D Betel, RM White, M Gershon, KG Margolis, L Studer
    Cell Stem Cell, 2023;30(3):264-282.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Modeling native and seeded Synuclein aggregation and related cellular dysfunctions in dopaminergic neurons derived by a new set of isogenic iPSC lines with SNCA multiplications
    Authors: A Iannielli, M Luoni, SG Giannelli, R Ferese, G Ordazzo, M Fossati, A Raimondi, F Opazo, O Corti, JHM Prehn, S Gambardell, R Melki, V Broccoli
    Cell Death & Disease, 2022-10-19;13(10):881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. A combined cell and gene therapy approach for homotopic reconstruction of midbrain dopamine pathways using human pluripotent stem cells
    Authors: N Moriarty, CW Gantner, CPJ Hunt, CM Ermine, S Frausin, S Viventi, DA Ovchinniko, D Kirik, CL Parish, LH Thompson
    Cell Stem Cell, 2022-02-17;29(3):434-448.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Hedgehog signaling is controlled by Rac1 activity
    Authors: C Tang, X Wu, Q Ren, M Yao, S Xu, Z Yan
    Theranostics, 2022-01-01;12(3):1303-1320.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons
    Authors: AM Earley, LF Burbulla, D Krainc, R Awatramani
    Scientific Reports, 2021-11-15;11(1):22257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease.
    Authors: de Rus Jacquet A, Tancredi J, Lemire A, DeSantis M, Li W, O'Shea E
    Elife, 2021-09-30;10(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Human stem cells harboring a suicide gene improve the�safety and standardisation of neural transplants in Parkinsonian rats
    Authors: IR de Luzy, KCL Law, N Moriarty, CPJ Hunt, JC Durnall, LH Thompson, A Nagy, CL Parish
    Nature Communications, 2021-05-27;12(1):3275.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3
    Authors: V Padmanabha, H Liu, G Ciceri, J Jungverdor, G Frishman, J Tchieu, GY Cederquist, I Rothenaign, K Schorpp, L Klepper, RM Walsh, TW Kim, D Cornacchia, A Ruepp, J Mayer, K Hadian, D Frishman, L Studer, M Vincendeau
    Cell Stem Cell, 2021-05-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys
    Authors: Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang
    Nature Medicine, 2021-03-01;0(0):.
    Species: Primate - Rhesus macaque
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use
    Authors: TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer
    Cell Stem Cell, 2021-02-04;28(2):343-355.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar
    Cell Stem Cell, 2021-02-04;28(2):217-229.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Defects in mRNA Translation in LRRK2-Mutant hiPSC-Derived Dopaminergic Neurons Lead to Dysregulated Calcium Homeostasis
    Authors: JW Kim, X Yin, A Jhaldiyal, MR Khan, I Martin, Z Xie, T Perez-Rose, M Kumar, L Abalde-Atr, J Xu, L Chen, SM Eacker, DJ Surmeier, NT Ingolia, TM Dawson, VL Dawson
    Cell Stem Cell, 2020-08-25;27(4):633-645.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020-06-19;27(1):125-136.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. The excretory/secretory products of fifth-stage larval�Angiostrongylus cantonensis induces autophagy via the Sonic hedgehog pathway in mouse brain astrocytes
    Authors: KY Chen, CJ Cheng, CC Cheng, KY Jhan, YJ Chen, LC Wang
    PLoS Negl Trop Dis, 2020-06-01;14(6):e0008290.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Erbb4 Is Required for Cerebellar Developmentand Malignant Phenotype of Medulloblastoma
    Authors: J Aldaregia, P Errarte, A Olazagoiti, M Gimeno, JJ Uriz, TR Gershon, I Garcia, A Matheu
    Cancers (Basel), 2020-04-17;12(4):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Stromal Hedgehog pathway activation by IHH suppresses lung adenocarcinoma growth and metastasis by limiting reactive oxygen species
    Authors: S Kasiri, B Chen, AN Wilson, A Reczek, S Mazambani, J Gadhvi, E Noel, U Marriam, B Mino, W Lu, L Girard, LM Solis, K Luby-Phelp, J Bishop, JW Kim, J Kim
    Oncogene, 2020-02-27;39(16):3258-3275.
    Species: Human
    Sample Types: Cells, Whole Cells
    Applications: Bioassay
  28. Metabolic and pathologic profiles of human LSS deficiency recapitulated in mice
    Authors: Y Wada, A Kikuchi, A Kaga, N Shimizu, J Ito, R Onuma, F Fujishima, E Totsune, R Sato, T Niihori, M Shirota, R Funayama, K Sato, T Nakazawa, K Nakayama, Y Aoki, S Aiba, K Nakagawa, S Kure
    PLoS Genet., 2020-02-26;16(2):e1008628.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  29. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets
    Authors: SC Vermilyea, A Babinski, N Tran, S To, S Guthrie, JH Kluss, JK Schmidt, GJ Wiepz, MG Meyer, ME Murphy, MR Cookson, ME Emborg, TG Golos
    Sci Rep, 2020-02-26;10(1):3447.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease
    Authors: CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish
    Cell Stem Cell, 2020-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen
    Cell Stem Cell, 2020;27(1):125-136.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Preparation and Co-Culture of iPSC-Derived Dopaminergic Neurons and Astrocytes.
    Authors: de Rus Jacquet A
    Curr Protoc Cell Biol, 2019-12-01;85(1):e98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors
    Authors: S Mahajani, A Raina, C Fokken, S Kügler, M Bähr
    Cell Death Dis, 2019-11-27;10(12):898.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  34. Immature mDA neurons ameliorate motor deficits in a 6-OHDA Parkinson's disease mouse model and are functional after cryopreservation
    Authors: D Leitner, M Ramamoorth, M Dejosez, TP Zwaka
    Stem Cell Res, 2019-10-24;41(0):101617.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  35. Isolation of LMX1a ventral midbrain progenitors improves the safety and predictability of human pluripotent stem cell-derived neural transplants in Parkinsonian Disease
    Authors: IR de Luzy, JC Niclis, CW Gantner, JA Kauhausen, CPJ Hunt, C Ermine, CW Pouton, LH Thompson, CL Parish
    J. Neurosci., 2019-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons
    Authors: M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard
    Cell Stem Cell, 2019-09-19;25(4):514-530.e8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Time series modeling of cell cycle exit identifies Brd4 dependent regulation of cerebellar neurogenesis
    Authors: C Penas, ME Maloof, V Stathias, J Long, SK Tan, J Mier, Y Fang, C Valdes, J Rodriguez-, CM Chiang, DJ Robbins, DJ Liebl, JK Lee, ME Hatten, J Clarke, NG Ayad
    Nat Commun, 2019-07-10;10(1):3028.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  38. hESC-Derived Thalamic Organoids Form Reciprocal Projections When Fused with Cortical Organoids
    Authors: Y Xiang, Y Tanaka, B Cakir, B Patterson, KY Kim, P Sun, YJ Kang, M Zhong, X Liu, P Patra, SH Lee, SM Weissman, IH Park
    Cell Stem Cell, 2019-02-21;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  39. Subtle Changes in the Levels of BCL-2 Proteins Cause Severe Craniofacial Abnormalities
    Authors: S Grabow, AJ Kueh, F Ke, HK Vanyai, BN Sheikh, MA Dengler, W Chiang, S Eccles, IM Smyth, LK Jones, FJ de Sauvage, M Scott, L Whitehead, AK Voss, A Strasser
    Cell Rep, 2018-09-18;24(12):3285-3295.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Identification of sonic hedgehog-regulated genes and biological processes in the cranial neural crest mesenchyme by comparative transcriptomics
    Authors: JL Everson, DM Fink, HM Chung, MR Sun, RJ Lipinski
    BMC Genomics, 2018-06-27;19(1):497.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. The COPII cargo adapter SEC24C is essential for neuronal homeostasis
    Authors: B Wang, JH Joo, R Mount, BJW Teubner, A Krenzer, AL Ward, VP Ichhaporia, EJ Adams, R Khoriaty, ST Peters, SM Pruett-Mil, SS Zakharenko, D Ginsburg, M Kundu
    J. Clin. Invest., 2018-06-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons�Recover Locomotive Defects of Nonhuman Primate Models of�Parkinson's Disease
    Authors: YK Wang, WW Zhu, MH Wu, YH Wu, ZX Liu, LM Liang, C Sheng, J Hao, L Wang, W Li, Q Zhou, BY Hu
    Stem Cell Reports, 2018-06-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. The Efficacy of Graphene Foams for Culturing Mesenchymal Stem Cells and Their Differentiation into Dopaminergic Neurons
    Authors: N Tasnim, V Thakur, M Chattopadh, B Joddar
    Stem Cells Int, 2018-06-03;2018(0):3410168.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons
    Authors: G Collo, L Cavalleri, M Zoli, U Maskos, E Ratti, E Merlo Pich
    Front Pharmacol, 2018-06-01;9(0):572.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. hPSC-derived Midbrain Dopaminergic Neurons Generated in a Scalable 3-D Biomaterial
    Authors: MM Adil, DV Schaffer
    Curr Protoc Stem Cell Biol, 2018-02-28;44(0):2D.21.1-2D.21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Ropinirole and Pramipexole Promote Structural Plasticity in Human iPSC-Derived Dopaminergic Neurons via BDNF and mTOR Signaling
    Authors: G Collo, L Cavalleri, F Bono, C Mora, S Fedele, RW Invernizzi, M Gennarelli, G Piovani, T Kunath, MJ Millan, E Merlo Pich, P Spano
    Neural Plast., 2018-02-04;2018(0):4196961.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  47. A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells
    Authors: JC Niclis, CW Gantner, CPJ Hunt, JA Kauhausen, JC Durnall, JM Haynes, CW Pouton, CL Parish, LH Thompson
    Stem Cell Reports, 2017-08-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
    Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
    PLoS Biol., 2017-08-04;15(8):e2002063.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Fusion of Regionally Specified hPSC-Derived Organoids Models Human Brain Development and Interneuron Migration
    Authors: Y Xiang, Y Tanaka, B Patterson, YJ Kang, G Govindaiah, N Roselaar, B Cakir, KY Kim, AP Lombroso, SM Hwang, M Zhong, EG Stanley, AG Elefanty, JR Naegele, SH Lee, SM Weissman, IH Park
    Cell Stem Cell, 2017-07-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Epigenetic and transcriptional modulation of WDR5, a chromatin remodeling protein, in Huntington's disease human induced pluripotent stem cell (hiPSC) model
    Authors: S Baronchell, A La Spada, A Ntai, A Barbieri, P Conforti, GS Jotti, S Redaelli, A Bentivegna, P De Blasio, I Biunno
    Mol. Cell. Neurosci., 2017-05-02;82(0):46-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017-01-18;541(7638):481-487.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Efficiently Specified Ventral Midbrain Dopamine Neurons from Human Pluripotent Stem Cells Under Xeno-Free Conditions Restore Motor Deficits in Parkinsonian Rodents
    Authors: JC Niclis, CW Gantner, WF Alsanie, SJ McDougall, CR Bye, AG Elefanty, EG Stanley, JM Haynes, CW Pouton, LH Thompson, CL Parish
    Stem Cells Transl Med, 2016-10-14;6(3):937-948.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Gli2 gene dosage and gene-environment interaction illuminate the etiological complexity of holoprosencephaly
    Dis Model Mech, 2016-09-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  54. BarTeL, a Genetically Versatile, Bioluminescent and Granule Neuron Precursor-Targeted Mouse Model for Medulloblastoma
    Authors: Gregory M Shacklefor
    PLoS ONE, 2016-06-16;11(6):e0156907.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  55. Explant culture of adult zebrafish hearts for epicardial regeneration studies
    Authors: J Cao, KD Poss
    Nat Protoc, 2016-04-07;11(5):872-81.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  56. Epicardial regeneration is guided by cardiac outflow tract and Hedgehog signalling.
    Authors: Wang J, Cao J, Dickson A, Poss K
    Nature, 2015-05-04;522(7555):226-30.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: Bioassay
  57. Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation.
    Authors: Shi Y, Chen J, Karner C, Long F
    Proc Natl Acad Sci U S A, 2015-03-30;112(15):4678-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015-03-23;6(0):6500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Selection Based on FOXA2 Expression Is Not Sufficient to Enrich for Dopamine Neurons From Human Pluripotent Stem Cells.
    Authors: Aguila J, Blak A, van Arensbergen J, Sousa A, Vazquez N, Aduriz A, Gayosso M, Lopez Mato M, Lopez de Maturana R, Hedlund E, Sonntag K, Sanchez-Pernaute R
    Stem Cells Transl Med, 2014-07-14;3(9):1032-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Impact of the Smoothened inhibitor, IPI-926, on smoothened ciliary localization and Hedgehog pathway activity.
    Authors: Peluso, Marisa O, Campbell, Veronica, Harari, Joseph A, Tibbitts, Thomas T, Proctor, Jennifer, Whitebread, Nigel, Conley, James M, White, Kerry F, Kutok, Jeffery, Read, Margaret, McGovern, Karen, Faia, Kerrie L
    PLoS ONE, 2014-03-07;9(3):e90534.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Lgr4 is a key regulator of prostate development and prostate stem cell differentiation.
    Authors: Luo W, Rodriguez M, Valdez J, Zhu X, Tan K, Li D, Siwko S, Xin L, Liu M
    Stem Cells, 2013-11-01;31(11):2492-505.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
    Authors: Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
    Cell Stem Cell, 2013-05-02;12(5):559-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Transcription factor-mediated reprogramming of fibroblasts to expandable, myelinogenic oligodendrocyte progenitor cells.
    Authors: Najm, Fadi J, Lager, Angela M, Zaremba, Anita, Wyatt, Krysta, Caprariello, Andrew V, Factor, Daniel C, Karl, Robert T, Maeda, Tadao, Miller, Robert H, Tesar, Paul J
    Nat Biotechnol, 2013-04-14;31(5):426-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Primary cilia attenuate hedgehog signalling in neoplastic chondrocytes.
    Authors: Ho, L, Ali, S A, Al-Jazrawe, M, Kandel, R, Wunder, J S, Alman, B A
    Oncogene, 2012-12-17;32(47):5388-96.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Tissue Culture
  65. Bax deficiency prolongs cerebellar neurogenesis, accelerates medulloblastoma formation and paradoxically increases both malignancy and differentiation.
    Authors: Garcia, I, Crowther, A J, Gama, V, Miller, C Ryan, Deshmukh, M, Gershon, T R
    Oncogene, 2012-06-18;32(18):2304-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  66. Specification of transplantable astroglial subtypes from human pluripotent stem cells.
    Authors: Krencik R, Weick JP, Liu Y, Zhang ZJ, Zhang SC
    Nat. Biotechnol., 2011-05-22;29(6):528-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.
    Authors: Zeng H, Guo M, Martins-Taylor K
    PLoS ONE, 2010-07-29;5(7):e11853.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  68. GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation.
    Authors: Nolan-Stevaux O, Lau J, Truitt ML, Chu GC, Hebrok M, Fernandez-Zapico ME, Hanahan D
    Genes Dev., 2009-01-01;23(1):24-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Levels of Gli3 repressor correlate with Bmp4 expression and apoptosis during limb development.
    Authors: Bastida MF, Delgado MD, Wang B, Fallon JF, Fernandez-Teran M, Ros MA
    Dev. Dyn., 2004-09-01;231(1):148-60.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. What is the difference between Recombinant Mouse Sonic Hedgehog Catalog # 464-SH and Catalog # 461-SH?

    • Recombinant Mouse Sonic Hedgehog, Catalog # 464-SH, possesses a N-terminal mutation that increases its potency in bioassay tests. The amino acid sequence is Cys25-Gly198 (Cys25Ile-Ile), accession number Q62226. The Cys25Ile-Ile mutation was created to match a publication that describes enhanced activity with these modifications: "http://www.ncbi.nlm.nih.gov/pubmed/11284692". Catalog # 461-SH does not possess this mutation.

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Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus
By Anonymous on 05/22/2017
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Used for patterning of iPSCs into neurons. Worked well.
Recombinant Mouse Sonic Hedgehog/Shh (C25II) N-Terminus
By Marco Onorati on 08/03/2016
Application: Stem/Immune cell maintenance or differentiation