Recombinant Mouse SorCS2 Protein, CF

Catalog #: 4237-SR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4237-SR-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Mouse SorCS2 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rmSorCS2 at 1 µg/mL (100 µL/well) can bind rh beta -NGF with an apparent K_D <75 nM.

Source

Mouse myeloma cell line, NS0-derived mouse SorCS2 protein
Ala118-Gly1078, with a C-terminal 6-His tag

Accession #

Q9EPR5

N-terminal Sequence
Analysis

Ala118

Predicted Molecular Mass

108.5 kDa

SDS-PAGE

120-125 kDa, reducing conditions

Product Datasheets

4237-SR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4237-SR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SorCS2

SorCS2 is a type I transmembrane glycoprotein receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in their specific locations (1 - 3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well-defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4 - 6). Mouse SorCS2 is synthesized as a 1159 amino acid (aa) preproform with a 49 aa signal sequence and a potential furin-type proteolytic processing site at aa 117. This would produce a mature SorCS2 protein of 1042 aa with a 961 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, mouse SorCS2 shares 98%, 89%, 85% and 76% aa identity with rat, human, equine and canine SorCS2, respectively. It also shares ~46% aa identity with the ECD of either SorCS1 or SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

References