Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Protein, CF

Catalog #: 10699-TM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10699-TM-050

Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera Protein Bioactivity.

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All TIM-1/KIM-1/HAVCR Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED₅₀ for this effect is 0.5-4.0 μg/mL.. Measured by its binding ability in a functional ELISA. When Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse TIM-4 (Catalog # 2826-TI) binds with an ED₅₀ of 0.8-6.4 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse TIM-1/KIM-1/HAVCR protein

Mouse TIM-1/KIM-1/HAVCR (Tyr22-Gly237) Accession # NP_599009.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_599009.2

N-terminal Sequence
Analysis

Tyr22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

75-85 kDa, under reducing conditions

Product Datasheets

10699-TM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10699-TM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) inhibits the proliferation of PHA-stimulated human T lymphoblasts. The ED₅₀ for this effect is 0.5‑4.0 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse TIM-1/KIM-1/HAVCR Isoform A Fc Chimera (Catalog # 10699-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa and 150-170 kDa, respectively.

Reconstitution Calculator

Background: TIM-1/KIM-1/HAVCR

TIM‑1 (T cell‑immunoglobulin‑mucin; also KIM‑1 and Tapr) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1, 2, 3, 4). This gene family is involved in the regulation of Th1 and Th2‑cell‑mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3 & 4) (1, 2). Mouse TIM‑1 and ‑2 are counterparts of human TIM‑1, while mouse TIM‑5 through TIM‑8 have no human counterparts (2). Mouse TIM‑1 (isoform 2) is synthesized as a 282 amino acid (aa) precursor that contains a 21 aa signal sequence, a 193 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V‑type Ig‑like domain and a mucin region characterized by multiple T‑S‑P motifs. The mucin region undergoes extensive O‑linked glycosylation. The mouse TIM‑1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). One isoform (termed isoform 1) possesses a 23 aa insertion after Pro182 (GenBank # NP_599099). Another splice variant (of isoform 1) shows a 15 aa deletion in the mucin region of the ECD (6). This difference is associated with a decreased susceptibility to asthma. In human, TIM‑1 is known to circulate as a soluble form that arises from cleavage by an undefined MMP, releasing an 85 ‑ 90 kDa soluble molecule (7). In mouse, a 60-65 kDa soluble form has also been detected (in urine) that presumably arises from proteolytic processing (8). In‑house data from R&D Systems Inc. has demonstrated the presence of soluble TIM‑1 in mouse circulation. The ECD of mouse TIM‑1 shares 37% and 81% aa sequence identity with human and rat TIM‑1 ECD, respectively. Reported ligands for TIM‑1 include TIM‑4, phosphatidylserine, P-Selectin and the hepatitis A virus (3, 9, 10, 11). TIM‑1 ligation induces T cell proliferation and promotes cytokine production (1, 12). In particular, it induces IL‑4 production, and requires the TIM‑1 cytoplasmic tyrosine phosphorylation motif (5). TIM-1 also serves as a cellular entry receptor for various viruses, including hepatitis A virus, Ebolavirus, Marburgvirus and has been indicated as a possible receptor for SARS-CoV-2 (9, 13, 14).

References

Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
Su, E.W. et al. (2008) Cytokine 44:9.
Freeman, G.J. et al. (2010) Immunol. Rev. 235:172.
Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
Herzog, C. et al. (2007) Kidney Int. 71:1009.
Feigelstock, D. et al. (1998) J. Virol. 72:6621.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Angiari, S. et al. (2014) J. Immuni. 40:542
Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Kondratowicz, A.S. et al. (2011) Proc. Natl. Acad. Sci. USA 108:8426.
Ichimura, T. et al. (2020) doi: 10.1101/2020.09.16.20190694. Preprint.

Long Name

T Cell Immunoglobulin Mucin-1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Alternate Names

CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1