Recombinant Mouse TINAGL1 Protein, CF Summary
Product Specifications
ED50 = 1-6 ng/mL.
Asp41-His466, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9208-TI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Mouse TINAGL1 (Catalog # 9208-TI) binds to Cultrex Mouse Laminin I in an ELISA Binding Assay. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an ED50 = 1-6 ng/mL.
Reconstitution Calculator
Background: TINAGL1
Tubulointerstitial Nephritis Antigen-like (TINAGL1), also known as Lipocalin-7, AZ-1, and Arg1, is an approximately 50 kDa matricellular protein that contains a Somatomedin-B like domain, a vWF-C domain, and a cysteine peptidase domain that lacks the critical active site Cys residue (1, 2). Mouse TINAGL1 shares 90% and 97% aa sequence identity with human and rat TINAGL1, respectively. It is a component of the extracellular matrix in basement membranes (3-5) where it binds to Collagens I and IV, Laminin, Integrins a5 and b1, and the Anastellin fragment of Fibronectin (3-5). TINAGL1 promotes cell adhesion and angiogenic sprouting from vascular endothelial cells (3, 6). It is expressed in the adrenal cortex and medulla, vascular smooth muscle cells, cardiac and skeletal muscle cells, and in blastocysts that are competent for implantation (2, 3, 5, 7).
- Kobayashi, M. et al. (2001) J. Steroid Biochem. Mol. Biol. 77:109.
- Wex, T. et al. (2001) Biochemistry 40:1350.
- Li, D. et al. (2007) FEBS Lett. 274:2506.
- Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
- Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
- Brown, L.J. et al. (2010) PLoS One 5:e13905.
- Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
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