Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF

Catalog #: 5330-RH Datasheet / COA / SDS

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5330-RH-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse TNFRH3/TNFRSF26 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse TRAIL/TNFSF10 (Catalog # 1121-TL) that produces 50% of the optimal binding response is approximately 15-75 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TNFRH3/TNFRSF26 protein

Mouse TNFRH3 (Ser20-Lys162) Accession # P83626	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

P83626

N-terminal Sequence
Analysis

Ser20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43.2 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

5330-RH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5330-RH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TNFRH3/TNFRSF26

Mouse TNFRH3 (Tumor necrosis factor receptor homolog 3; designated TNFR superfamily member 26) is a member of the TNF receptor superfamily. Northern blot analysis indicates its expression in the mouse spleen and thymus, and by a variety of mouse lymphoid cell lines (1). Mature mouse TNFRH3 is a 21 kDa (predicted, unglycosylated), 185 amino acid (aa) type I transmembrane protein that contains a 145 aa extracellular domain (ECD) and a 19 aa cytoplasmic domain. The ECD contains two potential sites of N‑linked glycosylation and three TNFR-Cys repeats that characterize TNF receptors, while the cytoplasmic portion lacks a death domain (1). No ortholog to mouse TNFRH3 has been reported in human or rat (1). Two TNFRH3‑related mouse TNF receptor homologs, DcTRAIL R1 (TNFRSF23/TNFRH1, 35% aa sequence identity within the ECD) and DcTRAIL R2 (TNFRSF22/TNFRH2, 40% aa sequence identity within the ECD) are classed as TRAIL decoy receptors. One report indicates that TNFRH3 binding of transfected TRAIL/TNFSF10 is below limits of detection in a cell-based assay, and it is thus thought not to serve as a TRAIL decoy receptor (1). Similarly, binding was not detected to any of 38 human and mouse TNF superfamily members in a cell‑based assay (2). However, TNFRH3 does bind recombinant trimeric mouse TRAIL in R&D Systems’ direct ELISA assay.

References

Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.

Long Name

Tumor Necrosis Factor Receptor Homolog 3

Entrez Gene IDs

244237 (Mouse)

Alternate Names

APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR; TNFRH3; Tnfrsf24; Tnfrsf26