Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Catalog #: 10172-TL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10172-TL-050

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED₅₀ for this effect is 0.45-4.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse TREML4/TLT-4 protein

Mouse TREML4/TLT-4 (Ser29-Leu197) Accession # Q3LRV9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q3LRV9

N-terminal Sequence
Analysis

Ser29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa

SDS-PAGE

64-72 kDa, under reducing conditions

Product Datasheets

10172-TL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10172-TL

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) binds to apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED₅₀ for this effect is 0.45‑4.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-72 kDa and 120-150 kDa, respectively.

Reconstitution Calculator

Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-like 4), also known as TLT-4, is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mature mouse TREML4 consists of a 172 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 42 aa cytoplasmic domain (1, 2). Within the ECD, mouse TREML4 shares 51% and 76% aa sequence identity with human and rat TREM-2, respectively. TREML4 is expressed in the spleen, CD8 alpha ⁺dendritic cells, and macrophages, and it is capable of recognizing necrotic cells by different phagocytes within the spleen (1). TREML4 is characterized by its Ig-like extracellular domain and short cytoplasmic tail that associates with adaptor DAP12 (2). TREML4 has been shown to play a role in coronary calcification and artery disease (3), and it is also a positive regulator of TLR7 signaling in autoimmunity and antiviral responses (4).

References