Recombinant Mouse TSH alpha/beta Heterodimer Protein
Recombinant Mouse TSH alpha/beta Heterodimer Protein Summary
Product Specifications
| Mouse TSH beta (Phe21-Val138) Accession # P12656 | (GGGS)3 | Mouse TSH alpha (Leu25-Ser120) Accession # P01216 |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8885-TH
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8885-TH/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: TSH alpha/beta Heterodimer
Thyroid Stimulating Hormone (TSH), also known as Thyrotropin, is a 28 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone, Luteinizing Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor (1-3). The approximately 14 kDa mouse CG alpha subunit shares 73% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The approximately 15 kDa mouse TSH beta subunit shares 89% and 92% aa sequence identity with the human and rat orthologs, respectively. Multiple isoforms of TSH with differing bioactivity and
half-lives exist due to differences in the post-translational glycosylation and sialylation modifications of its subunits (1, 4). TSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Thyrotropin-Releasing Hormone (TRH) from the hypothalamus. TSH travels to the thyroid gland and binds to the TSH Receptor to stimulate production of Thyroxine (T4), which is converted in peripheral tissues to Triiodothyronine (T3), a more biologically potent form of the thyroid hormone (1, 4, 5). TSH secretion is also controlled by plasma T3 and T4 via a negative feedback mechanism and by the neurotransmitters dopamine and Somatostatin (4, 5). Serum TSH levels are often assessed to evaluate thyroid function (4, 5). TSH has also been shown to be produced by bone marrow and is believed to have a direct effect on skeletal homeostasis by promoting bone strength and quality (6-9). Additionally, serum TSH levels have been shown to be decreased in the elderly (10).
- Szkudlinshi, M.W. et al. (2002) Physiol. Rev. 82:473.
- Farid, N.R. and M.W. Szkudlinski (2004) Endocrinology 145:4048.
- Cahoreau, C. et al. (2015) Front. Endocrinol. (Lausanne) 6:26.
- Roelfsema, F. and J.D. Veldhuis (2013) Endocr. Rev. 34:619.
- Winter, W.E. and M.R. Signorino (2001) Ann. Clin. Lab. Sci. 31:221.
- Klein, J.R. and H.C. Wang (2004) J. Exp. Biol. 207:55.
- Abe, E. et al. (2003) Cell 115:151.
- Sun, L. et al. (2006) Ann. N.Y. Acad. Sci. 1068:309.
- Sendak, R.A. et al. (2007) Int. Orthop. 31:753.
- Peeters, R.P. (2008) Hormones 7:28.
Citation for Recombinant Mouse TSH alpha/beta Heterodimer Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
Authors: S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser
EBioMedicine, 2021-10-11;72(0):103617.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse TSH alpha/beta Heterodimer Protein
There are currently no reviews for this product. Be the first to review Recombinant Mouse TSH alpha/beta Heterodimer Protein and earn rewards!
Have you used Recombinant Mouse TSH alpha/beta Heterodimer Protein?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
