Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF

Catalog # Availability Size / Price Qty
8948-VN-050

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Recombinant Mouse Vasorin/SLIT-like 2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse TGF- beta 2 (Catalog # 7346-B2) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse Vasorin/SLIT-like 2 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL
Source
Mouse myeloma cell line, NS0-derived mouse Vasorin/SLIT-like 2 protein
Cys25-Pro576, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys25
Predicted Molecular Mass
61 kDa
SDS-PAGE
72-97 kDa, reducing conditions

Product Datasheets

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8948-VN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8948-VN

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Ethylene Glycol and Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:


Reconstitution Calculator

Background: Vasorin/SLIT-like 2

Vasorin, also known as SLIT-like 2, is an approximately 110 kDa transmembrane protein that is expressed on vascular smooth muscle cells and in the developing skeletal system (1, 2). It is down-regulated following vascular injury (1). A 95 kDa soluble form of Vasorin can be shed from the cell surface by ADAM17 mediated cleavage (3). The shed soluble form of Vasorin binds to TGF-beta 1, 2, and 3 and prevents TGF-beta induced effects including intimal formation and epithelial mesenchymal transition (1, 3). Soluble Vasorin is elevated in the serum of hepatic carcinoma patients relative to normal or non-cancerous hepatic disease (4). It promotes tumor cell proliferation, migration, and survival (4). Mature mouse Vasorin consists of a 552 amino acid (aa) extracellular domain (ECD) with 10 leucine rich repeats (LRR) flanked by one LRRNT and one LRRCT domain, one EGF-like domain, and one Fibronectin type-III domain, a 21 aa transmembrane segment, and a 76 aa cytoplasmic domain (1). Within the ECD, mouse Vasorin shares 85% and 96% aa sequence identity with human and rat Vasorin, respectively.

References
  1. Ikeda, Y. et al. (2004) Proc. Natl. Acad. Sci. USA 101:10732.
  2. Krautzberger, A.M. et al. (2012) Gene Expr. Patterns 12:167.
  3. Malapeira, J. et al. (2011) Oncogene 30:1912.
  4. Li, S. et al. (2015) Oncotarget 6:10045.
Entrez Gene IDs
114990 (Human); 246154 (Mouse)
Alternate Names
Atia; Protein slit-like 2; SLIT like 2; Slitl2; slit-like 2 (Drosophila); slit-like 2; VASN; Vasorin

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