Recombinant Mouse Wnt-16b Protein

Catalog #: 9148-WN Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
9148-WN-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
9148-WN-025

Recombinant Mouse Wnt-16b Protein Bioactivity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-16b Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 2-12 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-16b protein
Asn30-Lys364

Accession #

Q9QYS1

N-terminal Sequence
Analysis

Asn30

Predicted Molecular Mass

38 kDa

SDS-PAGE

43-63 kDa, reducing conditions

Product Datasheets

9148-WN (with carrier)

Product Datasheet

COA

SDS

9148-WN/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9148-WN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9148-WN/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Wnt-16b (Catalog # 9148-WN) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells. The ED₅₀ for this effect is 2-12 µg/mL.

Reconstitution Calculator

Background: Wnt-16b

Wnt-16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine-rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature mouse Wnt‑16 shares 92% and 97% aa sequence identity with human and rat Wnt‑16, respectively. Wnt-16 is expressed by uterine stromal cells adjacent to the luminal epithelium during implantation (4). It is
up-regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt-16 in mouse embryos and human amniotic fluid (6). It is secreted by osteoblasts and inhibits monocyte differentiation into osteoclasts, thereby contributing to cortical bone thickness and bone mineral density [1279, 7]. Wnt-16 is over-expressed in cells undergoing replicative senescence, and it is up-regulated in articular cartilage by injury and osteoarthritis (9, 10). Wnt-16b expression in skin is up-regulated and enhances cell survival in human basal cell carcinomas (11). Its expression is also up-regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (12). Pre-B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A-Pbx1 fusion protein, also causes up-regulation of Wnt-16 that confers resistance to apoptosis (13, 14). Wnt-16 signaling through both canonical and JNK-mediated (non-canonical) pathways is reported (9-11).

References