Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF

Catalog #: 11200-WN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11200-WN-010

Recombinant Mouse Wnt-3/sFRP-1 Complex Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-3/sFRP-1 Complex Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 7.00-63.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3 protein

Mouse Wnt-3
(Gly22-Lys355)
Accession # P17553.1

Mouse sFRP-1 (Ser32-Lys314) Accession # Q8C4U3.3
N-terminus	C-terminus

Accession #

P17553.1, Q8C4U3.3

N-terminal Sequence
Analysis

Gly22 (Wnt-3) & Ser32 (sFRP-1)

Structure / Form

Non-covalent complex

Predicted Molecular Mass

37 kDa (Wnt-3) & 33 kDa (sFRP-1)

SDS-PAGE

36-45 kDa, under reducing conditions.

Product Datasheets

11200-WN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11200-WN

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Wnt-3/sFRP-1 Complex (Catalog # 11200-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 7.00-63.0 ng/mL.

Reconstitution Calculator

Background: Wnt-3

Wnt-3 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Mouse Wnt-3 shares 99% and 100% amino acid (aa) identity with human and rat Wnt-3, respectively. It also shares 87% aa identity with mouse Wnt3a. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (4). As intracellular beta -catenin levels rise, it binds to TCF/LEF transcription factors, leading to expression of Wnt target genes (5). Wnts are known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). Both sFRP-1 and sFRP-2 can inhibit Wnt-3a activity while sFRP-3 cannot (8). R&D Systems produces biologically active mouse Wnt-3 protein in a complex with mouse sFRP-1, and this Wnt-3/sFRP-1 complex is stable in the physiological buffer without detergent.

References

Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
Korinek, V. et al. (1997) Science 275:1784.
Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
Galli, L.M. et al. (2006) Dev. Dyn. 235:681.

Long Name

Wingless-type MMTV Integration Site Family, Member 3

Entrez Gene IDs

7473 (Human); 22415 (Mouse)

Alternate Names

INT4; INT4Proto-oncogene Int-4 homolog; MGC131950; MGC138321; MGC138323; proto-oncogene Wnt-3; TETAMS; wingless-type MMTV integration site family, member 3; WNT-3 proto-oncogene protein; WNT3; Wnt-3