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Recombinant Mouse Wnt-8a Protein

Catalog #: 8419-WN
3 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
8419-WN-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8419-WN-010
Wnt-8a Induces the Production of Alkaline Phosphatase.
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Product Details
Citations (3)
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Wnt-8a Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-8a protein
Ala20-Trp354
Accession #
NP_033316
N-terminal Sequence
Analysis
Ala20 & Trp23
Predicted Molecular Mass
37 kDa
SDS-PAGE
40-62 kDa, reducing conditions

Product Datasheets

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8419-WN (with carrier)

Product Datasheet
COA
SDS

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8419-WN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8419-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8419-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Wnt-8a Induces the Production of Alkaline Phosphatase. View Larger

Recombinant Mouse Wnt-8a stimulates alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 μg/mL.

SDS-PAGE Wnt-8a Induces the Production of Alkaline Phosphatase. View Larger

1 μg/lane of Recombinant Mouse Wnt-8a (Catalog # 8419-WN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 42.3 and 38.9 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-8a

Wnt-8a is a member of the large and highly conserved Wnt family of signaling molecules that have roles in pattern formation, cell fate decision, axon guidance, and tumor formation (1). Mouse Wnt-8a consists of a 19 amino acid (aa) signal peptide that is cleaved to release the mature 335 aa secreted protein (2). Mature mouse Wnt-8a shares 82% and 91% sequence identity with human and rat mature Wnt-8a, respectively. Similar to other canonical Wnts, Wnt-8a binds to the Frizzled family of receptors to intiate beta-catenin signaling. Wnt-8a is expressed during early embryogenesis and is involved in mesoderm patterning and posteriorization of the neuroectoderm (3-6). Wnt-8a signaling promotes optic lens development as well as otic placode formation during inner ear development (7, 8). Expression of Wnt-8a is observed in heart progenitor cells where it positively regulates cardiac myogenesis (9, 10).

References
  1. Anastas, J.N. and R.T. Moon (2013) Nat. Rev. Cancer 13:11.
  2. Bouillet, P. et al. (1996) Mech. Dev. 58:141.
  3. Rhinn, M. et al. (2005) Development 132:1261.
  4. Lekven, A.C. et al. (2001) Dev. Cell. 1:103.
  5. Erter, C.E. et al. (2001) Development 128:3571.
  6. Stump, R.J. et al. (2003) Dev. Biol. 259:48.
  7. Vendrell, V. et al. (2013) Mech. Dev. 130:160.
  8. Chong, C.C. et al. (2009) Exp. Eye Res. 88:307.
  9. Nakamura, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5834.
  10. Kwon, C. et al. (2007) Proc. Natl. Acad. Sci. USA 104:10894.
Long Name
Wingless-type MMTV Integration Site Family, Member 8a
Entrez Gene IDs
7478 (Human); 20890 (Mouse)
Alternate Names
protein Wnt-8a; Protein Wnt-8d; wingless-type MMTV integration site family, member 8A; Wnt8a; Wnt-8a; WNT8DWNT8d

Citations for Recombinant Mouse Wnt-8a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dysregulation of Wnt/?-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells
    Authors: Hao, J;Liu, C;Gu, Z;Yang, X;Lan, X;Guo, X;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Vangl2 promotes the formation of long cytonemes to enable distant Wnt/&beta-catenin signaling
    Authors: L Brunt, G Greicius, S Rogers, BD Evans, DM Virshup, KCA Wedgwood, S Scholpp
    Nature Communications, 2021-04-06;12(1):2058.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury
    Authors: CY Chang, MZ Liang, CC Wu, PY Huang, HI Chen, SF Yet, JW Tsai, CF Kao, L Chen
    Int J Mol Sci, 2020-02-21;21(4):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture

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