Recombinant Rabbit IL-21 Protein

Catalog #: 7274-RB Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
7274-RB-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7274-RB-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rabbit IL-21 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is ≤8.00 ng/mL.

Source

E. coli-derived rabbit IL-21 protein
His18-Arg150, with an N-terminal Met

Accession #

XP_008266192.1

N-terminal Sequence
Analysis

Met

Structure / Form

Monomer

Predicted Molecular Mass

15.5 kDa

SDS-PAGE

18 kDa, reducing conditions

Product Datasheets

7274-RB (with carrier)

Product Datasheet

COA

SDS

7274-RB/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7274-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7274-RB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-21

Interleukin‑21 (IL‑21) is an approximately 14 kDa four‑helix‑bundle cytokine in the family of cytokines that utilize the common gamma chain ( gamma _c) as a receptor subunit. gamma _c is also a subunit of the receptors for IL‑2, IL‑4, IL‑7, IL‑9, and IL‑15 (1). IL‑21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells (2‑6). It exerts its biological effects through a heterodimeric receptor complex of gamma _c and the IL‑21‑specific IL‑21 R (2, 7). Tfh‑derived IL‑21 plays an important role in the development of humoral immunity through its autocrine effects on the Tfh cell and paracrine effects on immunoglobulin affinity maturation, plasma cell differentiation, and B cell memory responses (4, 8, 9). It is also required for the migration of dendritic cells to draining lymph nodes (10). IL‑21 regulates several aspects of T cell function. It co‑stimulates the activation, proliferation, and survival of CD8⁺ T cells and NKT cells and promotes Th17 cell polarization (3, 5, 6, 11, 12). It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells (13, 14). IL‑21 R engagement enhances the cytolytic activity and IFN‑ gamma production of activated NK cells but limits the expansion of resting NK cells (15). In addition, IL‑21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation (16). Dysregulation of the IL‑21/IL‑21 R system contributes to the development of multiple immunological disorders (1, 17). The rabbit IL‑21 precursor contains a predicted 135 amino acid (aa) signal sequence and a 133 aa mature chain. Mature rabbit IL‑21 shares 55%‑67% aa sequence identity with mature canine, human, mouse, and rat IL‑21.

References