Recombinant Rat B7-1/CD80 Fc Chimera Protein, CF

Catalog #: 1214-B7 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1214-B7-050	In Stock	50 ug / $432

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat B7-1/CD80 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 0.1-0.4 µg/mL in the presence of PHA.

Source

Mouse myeloma cell line, NS0-derived rat B7-1/CD80 protein

Rat B7-1 (Ile39 - Gln248) Accession # AAB60503	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

AAB60503

N-terminal Sequence
Analysis

Ile39

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50.5 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

1214-B7

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1214-B7

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: B7-1/CD80

It is widely accepted that T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co-stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3 and PD-L2 (1). B7-1 is expressed on activated B cells, activated T cells, mature dendritic cells and macrophages (2). T cells express two different receptors capable of binding B7-1, CD28 and CTLA-4. CTLA-4 binds to B7-1 with a 20 - 100 fold higher affinity than CD28. Engagement of CD28 by B7-1 increases T cell proliferation and IL-2 production and enhances the immune response (3). In contrast, engagement of CTLA-4 is involved in the down-regulation of the immune response (4). Rat B7-1 is a 320 amino acid (aa) protein containing an extracellular domain, a transmembrane domain and a cytoplasmic domain. Rat and human B7-1 share 47% amino acid identity.

References

Coyle, A.J. and J-C. Gutierrez-Ramos (2001) Nature Immunol. 2:203.
Azuma, M. et al. (1992) Nature 366:76.
Gimmi, C.D. et al. (1991) Proc. Natl. Acad. Sci. USA 88:6575.
Walunas, T.L. et al. (1994) Immunity 1:405.

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Alternate Names

Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80