Recombinant Rat B7-2/CD86 Fc Chimera Protein, CF

Catalog #: 1340-B2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1340-B2-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat B7-2/CD86 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED₅₀ for this effect is 0.075-0.3 µg/mL in the presence of PHA.

Source

Mouse myeloma cell line, NS0-derived rat B7-2/CD86 protein

Rat B7-2 (Val29-Ile250) Accession # O35531	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

O35531

N-terminal Sequence
Analysis

Val29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

52 kDa (monomer)

SDS-PAGE

80-100 kDa, reducing conditions

Product Datasheets

1340-B2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1340-B2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: B7-2/CD86

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature rat B7-2 consists of a 222 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 19 aa transmembrane segment, and a 46 aa cytoplasmic tail (3). Within the ECD, rat B7-2 shares 59% and 81% aa sequence identity with human and mouse B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co‑stimulation (4, 5, 7, 9‑12). B7‑1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (4, 5, 7, 9‑11). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (13). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (11). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (14). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (15). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (16).

References

Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Maeda, K. et al. (1997) Int. Immunol. 9:993.
Azuma, M. et al. (1993) Nature 366:76.
Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
Hathcock, K.S. et al. (1993) Science 262:905.
Seino, K. et al. (1995) Int. Immunol. 7:1331.
Freeman, G.J. et al. (1993) Science 262:909.
Chen, C. et al. (1994) J. Immunol. 152:4929.
Lanier, L.L. et al. (1995) J. Immunol. 154:97.
Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
Wing, K. et al. (2011) Trends Immunol. 32:428.
Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
Kuchroo, V.K. et al. (1995) Cell 80:707.
Short, J.J. et al. (2006) Virus Res. 122:144.

Entrez Gene IDs

942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)

Alternate Names

Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86