Recombinant Rat BMP-6 Protein, CF

Catalog #: 10420-BM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10420-BM-020

Recombinant Rat BMP-6 Protein Bioactivity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat BMP-6 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived rat BMP-6 protein
Ser368-His506

Accession #

Q04906.2

N-terminal Sequence
Analysis

Ser368

Structure / Form

Covalent dimer

Predicted Molecular Mass

16 kDa

SDS-PAGE

16-22 kDa, under reducing conditions

Product Datasheets

10420-BM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10420-BM

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 200 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Rat BMP-6 (Catalog # 10420-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED₅₀ for this effect is 0.15-0.9 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Rat BMP-6 (Catalog # 10420-BM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-22 kDa and 32-44 kDa, respectively.

Reconstitution Calculator

Background: BMP-6

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, is a member of the BMP subfamily of TGF-beta superfamily proteins. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis (1). BMP-6 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release a smaller, C-terminal mature protein. Biologically active BMP-6 consists of a disulfide-linked homodimer of the mature protein, with each monomer containing a characteristic cystine knot motif (2). Mature rat BMP-6 shares 98% and 96% amino acid sequence identity with human and mouse BMP-6, respectively. Cellular responses to BMP-6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3-5). BMP-6 induces the expression of Noggin and is subsequently antagonized by Noggin (6). BMP-6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (7), chondrocyte maturation (8), Ang II-induced aldosterone production in the adrenal cortex (4), hormone production and responsiveness in ovarian granulosa cells (9), iNOS and TNF-alpha production in macrophages (5), the cell death of B cells (10), and neurite outgrowth (11). BMP-6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). It enhances tumor progression by promoting local angiogenesis and differentiation of immune tolerizing M2 macrophages (13-15). Through interactions with the BMP co-receptor RGM-C/Hemojuvelin, BMP-6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (16). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (3).

References

Bragdon, B. et al. (2010) Cell Signal. 23:609.
Allendorph GP et al. (2007) Biochemistry. 46:12238.
Israel, D.I. et al. (1996) Growth Factors 13:291.
Inagaki, K. et al. (2006) Endocrinology 147:2681.
Hong, J.H. et al. (2008) Immunology 128:e442.
Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
Shi, J. et al. (2009) Fertil. Steril. 92:1794.
Kersten, C. et al. (2005) BMC Immunol. 6:9.
Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
Zhang, Z. et al. (2006) Neuroscience 138:47.
Dai, J. et al. (2005) Cancer Res. 65:8274.
Kwon, S.J. et al. (2014) Prostate 74:121.
Lee, J.-H. et al. (2013) Cancer Res. 73:3604.
Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.

Long Name

Bone Morphogenetic Protein 6

Entrez Gene IDs

654 (Human); 12161 (Mouse); 25644 (Rat)

Alternate Names

BMP6; BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1