Recombinant Rat CD155/PVR His-tag Protein, CF

Catalog #: 9764-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9764-CD-050

Recombinant Rat CD155/PVR His-tag Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CD155/PVR His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat CD155/PVR is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 (Catalog # 5690-CD) that produces 50% optimal binding response is 1.5-7.5 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived rat CD155/PVR protein
Glu34-Leu351, with a C-terminal 6-His tag

Accession #

NP_058772

N-terminal Sequence
Analysis

Glu34

Predicted Molecular Mass

35 kDa

SDS-PAGE

62-71 kDa, reducing conditions

Product Datasheets

9764-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9764-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Rat CD155/PVR (Catalog # 9764-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CD96 (Catalog # 5690-CD) binds with an ED₅₀ of 1.5-7.5 ng/mL.

Reconstitution Calculator

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). The protein may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3, 4). Mature rat CD155/PVR consists of three Ig-like domains and a 21-amino acid (aa) transmembrane segment. Within the ECD, rat CD155/PVR shares 44% and 73% aa sequence identity with human and mouse CD155/PVR, respectively. CD155/PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155/PVR is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155/PVR expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM-1 to endothelial cell PVR promotes transendothelial migration (7).

References