Recombinant Rat CD30/TNFRSF8 Fc Chimera Protein, CF

Catalog #: 11034-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11034-CD-100

Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CD30/TNFRSF8 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD30 Ligand/TNFSF8 (Catalog # 732-CL/CF) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera binds with and ED₅₀ of 20.0-120 ng/mL.

Source

Mouse myeloma cell line, NS0-derived rat CD30/TNFRSF8 protein

Rat CD30 (Phe19-Leu255) Accession # EDL81069.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

EDL81069.1

N-terminal Sequence
Analysis

Phe19

Structure / Form

Disulfide linked homodimer

Predicted Molecular Mass

52 kDa

SDS-PAGE

80-95 kDa, under reducing conditions.

Product Datasheets

11034-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11034-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse CD30 Ligand/TNFSF8 (732-CL) is immobilized at 0.250 μg/mL (100 μL/well), Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein (Catalog # 11034-CD) binds with and ED₅₀ of 20.0-120 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Rat CD30/TNFRSF8 Mouse Fc Chimera Protein (Catalog # 11034-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-95 kDa and 160-190 kDa, respectively.

Reconstitution Calculator

Background: CD30/TNFRSF8

Lymphocyte activation antigen CD-30 (CD30), also known as Tumor necrosis factor receptor superfamily member 8 (TNFRSF8), is a type I transmembrane glycoprotein belonging to the TNFR superfamily (1, 2). Mature rat CD30 consists of an extracellular domain (ECD) containing four disulfide bonds and two cysteine-rich repeats, a transmembrane domain, and a cytoplasmic domain. Within the ECD, rat CD30 shares 45% and 79% amino acid (aa) sequence identity with human and mouse CD30, respectively. In human, isoforms of CD30 with truncated cytoplasmic domains can be generated as well as a soluble form, which is produced by proteolytic cleavage of CD30 at the cell surface and is found in serum (3, 4). CD30 is normally expressed on antigen-stimulated Th cells and B cells (5 ‑ 7). CD30 binds to CD30 Ligand/TNFSF8 which is expressed on activated Th cells, monocytes, granulocytes and medullary thymic epithelial cells (2, 6). Signaling via CD30‑CD30L has been shown to induce the activation and proliferation of T cells and the CD30–CD30L pathway has been implicated as a major player in secondary humoral immune responses (2). In the absence of antigenic stimulation, CD30 can still induce T cell expression of IL‑13 (8). CD30 contributes to thymic negative selection by inducing the apoptotic cell death of CD4+CD8+ T cells (8, 9). In B cells, CD30 ligation promotes cellular proliferation and antibody production in addition to the expression of CXCR4, CCL3, and CCL5 (6, 10). Soluble CD30 retains the ability to bind CD30 Ligand and functions as an inhibitor of normal CD30 signaling (11). CD30 is up‑regulated in Hodgkin's disease (on Reed-Sternberg cells), other lymphomas, chronic inflammation, and autoimmunity (12).

References

Hehlgans, T. & Pfeffer, K. (2005) Immunology 115(1):1–20.
Kennedy, M.K. et al. (2006) Immunology 118:143.
van der Weyden, C.A. et al. (2017). Blood Cancer J. 7:e603
Buchan, S.L. and Al-Shamkhani, A. (2012) PLoS ONE 7:e45244.
Hamann, D. et al. (1996) J. Immunol. 156:1387.
Shanebeck, S.D. et al. (1995) Eur. J. Immunol. 25:2147.
Gruss, H.-J. et al. (1994) Blood 83:2045.
Harlin, H. et al. (2002) J. Immunol. 169:2451.
Amakawa, R. et al. (1996) Cell 84:551.
Vinante, F. et al. (2002) Blood 99:52.
Hargreaves, P.G. and A. Al-Shamkhani (2002) Eur. J. Immunol. 32:163.
Oflzoglu E. et al. (2009) Adv. Exp. Med. Biol. 647:174.

Entrez Gene IDs

943 (Human); 21941 (Mouse); 25069 (Rat)

Alternate Names

CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8