Recombinant Rat CLEC10A/CD301 Protein, CF

Catalog #: 9037-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9037-CL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Rat CLEC10A/CD301 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat CLEC10A/CD301 is immobilized at 2.5 µg/mL (100 µL/well), the concentration of biotinylated Recombinant Viral EBOV GP that produces 50% of the optimal binding response is approximately 1-6 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived rat CLEC10A/CD301 protein
Gln59-Ser306, with an N-terminal 6-His tag

Accession #

NP_071788

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

29 kDa

SDS-PAGE

34-42 kDa, reducing conditions

Product Datasheets

9037-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9037-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein in the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Rat CLEC10A consists of a 37 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment, and a 248 aa extracellular domain (ECD) with one carbohydrate recognition domain and a neck region (2). Within the ECD, rat CLEC10A shares 56% and 79% aa sequence identity with human CLEC10A and mouse CLEC10A/MGL1, respectively. CLEC10A is expressed on immature myleloid dendritic cells, Langerhans cells, and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-8). It is up-regulated on pro-inflammatory monocytes in obesity and promotes their trafficking to adipose tissue (9). CLEC10A selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates including the Tn carcinoma antigen (3, 4, 10-12). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (13). CLEC10A expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) on T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (14).

References

Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
Ii, M. et al. (1990) J. Biol. Chem. 265:11295.
Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
Valladeau, J. et al. (2001) J. Immunol. 167:5767.
van Vliet, S.J. et al. (2006) Immunobiology 211:577.
van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
Dupasquier, M. et al. (2006) J. Leukoc. Biol. 80:838.
Westcott, D.J. et al. (2009) J. Exp. Med. 206:3143.
Suzuki, N. et al. (1996) J. Immunol. 156:128.
van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
Higashi, N. et al. (2002) Int. Immunol. 14:545.
Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
Takada, A. et al. (2004) J. Virol. 78:2943.

Long Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

Entrez Gene IDs

10462 (Human)

Alternate Names

CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLEC10A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL