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Recombinant Rat CTGF Protein, CF

Catalog #: 9237-CT
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9237-CT-050
Recombinant Rat CTGF Protein Bioactivity
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Product Details
Citations (7)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat CTGF Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. The ED50 for this effect is 1-5 μg/mL
Source
Human embryonic kidney cell, HEK293-derived rat CTGF/CCN2 protein
Gln25-Ala347
Accession #
Q9R1E9
N-terminal Sequence
Analysis
No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Asp26
Predicted Molecular Mass
35 kDa
SDS-PAGE
35-41 kDa, reducing conditions

Product Datasheets

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9237-CT

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9237-CT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Rat CTGF Protein Bioactivity View Larger

Immobilized Recombinant Rat CTGF (Catalog # 9237-CT) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 μg/mL

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Rat CTGF shares 94% and 99% amino acid (aa) sequence identity with human and mouse CTGF, respectively. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).

References
  1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Xu, J. et al. (2000) J. Cell. Biochem. 77:103.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
  4. Schober, J.M. et al. (2002) Blood 99:4457.
  5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
  6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
  7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
  8. Mercurio, S. et al. (2004) Development 131:2137.
  9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
  10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
  11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
  12. Khodosevich, K. et al. (2013) Neuron 79:1136.
  13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
  14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
  15. Canalis, E. et al. (2010) Endocrinology 151:3490.
  16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
  17. Ivkovic, S. et al. (2003) Development 130:2779.
  18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
  19. Shimo, T. et al. (1999) J. Biochem. 126:137.
Long Name
Connective Tissue Growth Factor
Entrez Gene IDs
1490 (Human); 14219 (Mouse); 64032 (Rat)
Alternate Names
CCN2; CCN2IGFBP-8; connective tissue growth factor; CTGF; CTGRP; Fisp12; HCS24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8CCN family member 2; Insulin-like growth factor-binding protein 8; MGC102839; NOV2

Citations for Recombinant Rat CTGF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Rescue of murine hind limb ischemia via angiogenesis and lymphangiogenesis promoted by cellular communication network factor 2
    Authors: Shimizu, M;Yoshimatsu, G;Morita, Y;Tanaka, T;Sakata, N;Tagashira, H;Wada, H;Kodama, S;
    Scientific reports
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. CTGF promotes the repair and regeneration of alveoli after acute lung injury by promoting the proliferation of subpopulation of AEC2s
    Authors: Sun, J;Zhang, H;Liu, D;Liu, W;Du, J;Wen, D;Li, L;Zhang, A;Jiang, J;Zeng, L;
    Respiratory research
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Structure, signals, and cellular elements of the mouse gastric mesenchymal niche
    Authors: E Manieri, G Tie, D Seruggia, S Madha, A Maglieri, K Huang, Y Fujiwara, K Zhang, S Orkin, R He, N McCarthy, RA Shivdasani
    bioRxiv : the preprint server for biology, 2023-02-24;0(0):.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  4. Glycocholic acid aggravates liver fibrosis by promoting the up-regulation of connective tissue growth factor in hepatocytes
    Authors: Z Yuan, J Wang, H Zhang, Y Chai, Y Xu, Y Miao, Z Yuan, L Zhang, Z Jiang, Q Yu
    Cellular Signalling, 2022-10-29;101(0):110508.
    Species: Rat
    Sample Types:
    Applications: Bioassay
  5. CCN2-induced lymphangiogenesis is mediated by the integrin alphavbeta5-ERK pathway and regulated by DUSP6
    Authors: S Hashiguchi, T Tanaka, R Mano, S Kondo, S Kodama
    Scientific Reports, 2022-01-18;12(1):926.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  6. A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction
    Authors: B Sun, M Lian, Y Han, X Mo, W Jiang, Z Qiao, K Dai
    Bioact Mater, 2020-08-14;6(1):179-190.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Fluvastatin inhibits advanced glycation end products-induced proliferation, migration, and extracellular matrix accumulation in vascular smooth muscle cells by targeting connective tissue growth factor
    Authors: AR Hwang, JO Nam, YJ Kang
    Korean J. Physiol. Pharmacol., 2018-02-23;22(2):193-201.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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