Recombinant Rat IL-1 RI Fc Chimera Protein, CF

Catalog #: 4895-MR
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4895-MR-100

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-1 RI Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. Approximately 5‑20 µg/mL of rrIL-1 RI/Fc Chimera will inhibit 50% of the biological response due to 15 pg/mL of rrIL-1a.

Source

Mouse myeloma cell line, NS0-derived rat IL-1 RI protein

Rat IL-1 RI (Leu20 - Lys338) Accession # Q02955	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

Q02955

N-terminal Sequence
Analysis

Leu20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

64.3 kDa (monomer)

SDS-PAGE

85-100 kDa, reducing conditions

Product Datasheets

4895-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4895-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-1 RI

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily (1 - 3). IL-1 RI is an 80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein (1, 2). This complex recruits the adaptor protein MyD88, to initiate signaling in the NF kappa B pathway (4, 5). Rat IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Rat IL-1 RI shares 83%, 65%, 60%, 60% and 54% aa identity with mouse, human, canine, equine and bovine IL-1 RI, respectively. Two additional splice isoforms of rat IL-1 RI have been described (6). One has an N-terminus that is extended by 14 amino acids, but appears to have equivalent function. The other lacks the TM sequence and is secreted as an ~82 kDa protein that antagonizes the effect of IL-1 beta. Thus, the role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and inhibitory IL-1 R isoforms and decoys (1 - 6). IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever (1, 2, 5, 7).

References

Boraschi, D. & A. Tagliabue (2006) Vitam. Horm. 74:229.
Dinarello, C.A. (2002) Clin. Exp. Rheumatol. 20:S1.
Hart, R.P. et al. (1993) J. Neuroimmunol. 44:49.
Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.
Gasse, P. et al. (2007) J. Clin. Invest. 117:3786.
Yamada, M. et al. (2007) Nitric Oxide 17:98.
Ching, S. et al. (2007) J. Neurosci. 27:10476.

Long Name

Interleukin 1 Receptor I

Entrez Gene IDs

3554 (Human); 16177 (Mouse)

Alternate Names

CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80

Related Research Areas

Citation for Recombinant Rat IL-1 RI Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

P2X7 receptor inhibition attenuated sympathetic nerve sprouting after myocardial infarction via the NLRP3/IL-1? pathway
Authors: J Yin, Y Wang, H Hu, X Li, M Xue, W Cheng, Y Wang, X Li, N Yang, Y Shi, S Yan
J. Cell. Mol. Med., 2017-05-04;0(0):.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo