Recombinant Rat IL-17F Protein

Catalog #: 4437-IL Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
4437-IL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
4437-IL-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-17F Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to compete with immobilized rmIL-17F for binding with rmIL-17R in a functional ELISA.

Source

E. coli-derived rat IL-17F protein
Ala27-Ala161

Accession #

NP_001015011.2

N-terminal Sequence
Analysis

Ala19

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

15.1 kDa (monomer)

Product Datasheets

4437-IL (with carrier)

Product Datasheet

COA

SDS

4437-IL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4437-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4437-IL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-17F

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa identical with mature human and mouse IL-17F, respectively; it also shows 55% aa identity to rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated TH17-type CD4+ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta, IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma, IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

References