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Recombinant Rat IL-18/IL-1F4 Protein

Catalog #: 521-RL
14 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
521-RL-025/CF

With Carrier

Catalog # Availability Size / Price Qty
521-RL-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (14)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-18/IL-1F4 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce mouse IFN-gamma secretion by activated mouse T cells. The ED50 for this effect is 10.0-60.0 ng/mL.
Source
E. coli-derived rat IL-18/IL-1F4 protein
His37-Ser194, with an N-terminal Met
Accession #
P97636.1
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.4 kDa

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521-RL (with carrier)

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SDS

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521-RL/CF (carrier free)

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COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

521-RL

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, EDTA, TCEP and PEG 8000 with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

521-RL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, EDTA, TCEP and PEG 8000 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature rat IL-18 shares 62% and 91% amino acid sequence identity with human and mouse IL-18, respectively (15). Alternative splicing generates an isoform with a 19 aa internal deletion.

References
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Culhane, A.C. et al. (1998) Mol. Psychiatry 3:362.
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Citations for Recombinant Rat IL-18/IL-1F4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Intestinal IL-22RA1 signaling regulates intrinsic and systemic lipid and glucose metabolism to alleviate obesity-associated disorders
    Authors: Gaudino, SJ;Singh, A;Huang, H;Padiadpu, J;Jean-Pierre, M;Kempen, C;Bahadur, T;Shiomitsu, K;Blumberg, R;Shroyer, KR;Beyaz, S;Shulzhenko, N;Morgun, A;Kumar, P;
    Nature communications
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  2. Exploring the application of sildenafil for high-fat diet-induced erectile dysfunction based on interleukin-18-mediated NLRP3/Caspase-1 signaling pathway
    Authors: Zhu, B;Niu, Y;Niu, L;Zhang, X;Liu, F;
    Sexual medicine
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo assay
  3. Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network
    Authors: Lilley, RJ;Taylor, KD;Wildman, SSP;Peppiatt-Wildman, CM;
    Frontiers in physiology
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Rapamycin protects chondrocytes against IL-18-induced apoptosis and ameliorates rat osteoarthritis
    Authors: J Bao, Z Chen, L Xu, L Wu, Y Xiong
    Aging (Albany NY), 2020-03-17;12(6):5152-5167.
    Species: Rat
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  5. Costunolide inhibits matrix metalloproteinases expression and osteoarthritis via the NF??B and Wnt/??catenin signaling pathways
    Authors: Y He, SAA Moqbel, L Xu, J Ran, C Ma, K Xu, J Bao, L Jiang, W Chen, Y Xiong, L Wu
    Mol Med Rep, 2019-05-14;0(0):.
    Species: Rat
    Sample Types: Primary Cells
    Applications: Cell Culture
  6. Wnt/?-catenin and Hedgehog pathways are involved in the inflammatory effect of Interleukin 18 on rat chondrocytes
    Authors: J Bao, C Ma, J Ran, Y Xiong, S Yan, L Wu
    Oncotarget, 2017-08-24;8(66):109962-109972.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Frontline Science: A hyporesponsive subset of rat NK cells negative for Ly49s3 and NKR-P1B are precursors to the functionally mature NKR-P1B(+) subset
    Authors: A Sudworth, JT Vaage, M Inngjerdin, L Kveberg
    J. Leukoc. Biol., 2017-07-26;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Interleukin-18 gene deletion protects against sepsis-induced cardiac dysfunction by inhibiting PP2A activity
    Authors: Y Okuhara, S Yokoe, T Iwasaku, A Eguchi, K Nishimura, W Li, M Oboshi, Y Naito, T Mano, M Asahi, H Okamura, T Masuyama, S Hirotani
    Int. J. Cardiol., 2017-05-04;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Authors: M Boieri, A Ulvmoen, A Sudworth, C Lendrem, M Collin, AM Dickinson, L Kveberg, M Inngjerdin
    Oncoimmunology, 2017-01-17;6(3):e1274478.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Immunoglobulins stimulate cultured Schwann cell maturation and promote their potential to induce axonal outgrowth.
    Authors: Tzekova N, Heinen A, Bunk S, Hermann C, Hartung H, Reipert B, Kury P
    J Neuroinflammation, 2015-05-29;12(0):107.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade.
    Authors: Guo W, Miyoshi K, Dubner R, Gu M, Li M, Liu J, Yang J, Zou S, Ren K, Noguchi K, Wei F
    Mol Pain, 2014-06-09;10(0):35.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  12. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
    Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
    J. Neurosci., 2008-11-26;28(48):12775-87.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  13. Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis.
    Authors: Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE
    Arthritis Rheum., 2006-08-01;54(8):2402-14.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  14. Role of IL-18 in acute lung inflammation.
    Authors: Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA
    J. Immunol., 2001-12-15;167(12):7060-8.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA (Standard)

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