Recombinant Rat IL-7 R alpha Fc Chimera Protein, CF

Catalog #: 5607-RR Datasheet / COA / SDS

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5607-RR-050

R&D Systems Recombinant Proteins and Enzymes

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All IL-7R alpha/CD127 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat IL-7 R alpha Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When 25 ng/mL of rrIL-7R alpha /Fc Chimera is captured by goat anti-mouse IgG Fc Chimera (Catalog # G-202-C) coated plate, the concentration of rmIL-7 that produces 50% optimal binding response is found to be approximately 10-30 ng/mL.

Source

Mouse myeloma cell line, NS0-derived rat IL-7 R alpha/CD127 protein

Rat IL-7R alpha (Glu21 - Asp239) Accession # NP_001099888	IEGRMDP	Mouse IgG_2a (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

NP_001099888

N-terminal Sequence
Analysis

Glu21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

52.2 kDa (monomer)

SDS-PAGE

60-75 kDa under reducing conditions

Product Datasheets

5607-RR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5607-RR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature rat IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3, Accession # NP_001099888). Within the ECD, rat IL-7 R alpha shares 67% and 79% aa sequence identity with human and mouse IL-7 R alpha, respectively. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
Goodwin, R.G. et al. (1990) Cell 60:941.
Noguchi, M. et al. (1993) Science 262:1877.
Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
Jahn, T. et al. (2007) Blood 110:1840.
Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Alternate Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha