Recombinant Rat LILRC1 Protein, CF

Catalog #: 9698-T4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9698-T4-050

Recombinant Rat LILRC1 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat LILRC1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat LILRC1 is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED₅₀ of 0.05-0.3 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat LILRC1 protein
Gln17-Asn248, with a C-terminal 6-His tag

Accession #

NP_001070261

N-terminal Sequence
Analysis

No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Glu18

Predicted Molecular Mass

27 kDa

SDS-PAGE

34-41 kDa, reducing conditions

Product Datasheets

9698-T4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9698-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Rat LILRC1 (Catalog # 9698-T4) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED₅₀ of 0.05-0.3 µg/mL.

Reconstitution Calculator

Background: LILRC1

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRC1 (leukocyte immunoglobulin-like receptor C1), also known as LILRA5, ILT11, LIR-9, and CD85f, consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic tail (2, 3). The ECD contains two Ig-like domains (4), and the transmembrane segment contains a positively charged arginine residue which may mediate its association with the signaling molecule, FcR common gamma chain (3, 5). Within the ECD, rat LILRC1 shares 58% and 85% amino acid sequence identity with human and mouse LILRA5, respectively. LILRC1 is expressed by monocytes, macrophages, and neutrophils (2, 3, 5). It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients (2, 5). Cross-linking of LILRC1 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10 (2, 5). R&D Systems in-house testing indicates that LILRC1/LILRA5 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References