Recombinant Rat LILRC2 Protein, CF

Catalog #: 9808-T4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9808-T4-050

Recombinant Rat LILRC2 Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat LILRC2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat LILRC2 is immobilized at 2 μg/mL,100 μL/well, the concentration of Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) that produces 50% of the optimal binding response is 0.04-0.24 μg/mL

Source

Mouse myeloma cell line, NS0-derived rat LILRC2 protein
Met1-Asn230, with a C-terminal 6-His tag

Accession #

AAY44812

N-terminal Sequence
Analysis

Gly24

Predicted Molecular Mass

24 kDa

SDS-PAGE

32-43 kDa, reducing conditions

Product Datasheets

9808-T4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9808-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Rat LILRC2 (Catalog # 9808-T4) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED₅₀ of 0.04-0.24 µg/mL.

Reconstitution Calculator

Background: LILRC2

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRC2 (leukocyte immunoglobulin-like receptor C2) is a 35 kDa member of the LILRs that are expressed on hematopoietic cells. Mature rat LILRC2 is a type I transmembrane (TM) protein that is 241 amino acids (aa) in length. It contains a 207 aa extracellular domain (ECD), a 19 aa TM segment, and a short cytoplasmic tail (2). The ECD contains two Ig-like domains while the TM segment possesses an Arg residue (2, 3). This suggests that LILRC2 is an activating receptor that may interact with Fc epsilon RI gamma and/or CD3 zeta. Cells expressing LILRC2 include neutrophils, B cells, and CD4⁺ and CD8⁺ T cells (2). No human or mouse LILRC2 is known. Rat LILRC2 shares 47.9% identity with LILRC1. R&D Systems in-house testing indicates that LILRC2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (4).

References