Recombinant Rat Neurexophilin-1/NXPH1 Protein, CF Summary
Product Specifications
Ala22-Gly271 & Arg107-Gly271, both with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4208-NX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Neurexophilin-1
Neurexophilin-1 (NXPH-1) is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family (1, 2). The 29 kDa, 271 amino acid (aa) NXPH-1 sequence contains a 22 aa signal peptide, a 94 aa propeptide that is cleaved at a basic motif, and a 115 aa mature protein that contains three potential N-glycosylation sites in the N-terminal portion and six conserved cysteines in the C-terminal portion (1). Mature rat NXPH-1 shares 99%, 99%, 99%, 96% and 84% aa identity with mouse, human, bovine, opossum and zebrafish NXPH-1, respectively. NXPH-1 is expressed selectively in subpopulations of neurons within the cerebral cortex, cerebellum and olfactory bulb that are thought likely to be inhibitory interneurons (2, 3). NXPH-3 is the neurexophilin most similar to NXPH-1, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to coincide, but both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 4, 5). Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination (4, 6). Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins (1, 4).
- Missler, M. and T. C. Sudhof (1998) J. Neurosci. 18:3630.
- Petrenko, A. G. et al. (1996) J. Neurosci. 16:4360.
- Clarris, H. J. et al. (2002) Int. J. Dev. Biol. 46:649.
- Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
- Dudanova, I. et al. (2006) J. Neurosci. 26:10599.
- Beglopoulos, V. et al. (2005) Mol. Cell. Biol. 25:7278.
Citation for Recombinant Rat Neurexophilin-1/NXPH1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells.
Authors: Kinzfogl J, Hangoc G, Broxmeyer HE
Blood, 2011-05-31;118(3):565-75.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo
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