Recombinant Rat Neurexophilin-3/NXPH3 Protein, CF

Catalog #: 5098-NX Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5098-NX-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Rat Neurexophilin-3/NXPH3 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a competitive binding assay. When rrNeurexin-1 alpha (Catalog # 4485-NX) is immobilized at 1 µg/mL (100 µL/well), rrNeurexophilin-3 inhibits 50% binding of biotinylated rrNeurexophilin-1 (1 µg/mL) at the concentration range of 1‑3 µg/mL.

Source

Mouse myeloma cell line, NS0-derived rat Neurexophilin-3 protein
Gln23-Gly252, with a C-terminal 6-His tag

Accession #

AAI66603

N-terminal Sequence
Analysis

No results obtained: Gln23 predicted

Predicted Molecular Mass

26.5 kDa

SDS-PAGE

40-43 kDa, reducing conditions

Product Datasheets

5098-NX

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5098-NX

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Neurexophilin-3

Neurexophilin-3 (NXPH3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family (1, 2). The 252 amino acid (aa) NXPH3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein (1). Mature rat NXPH3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion (1). NXPH3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development (3). NXPH1 is the neurexophilin most similar to NXPH3, sharing 69% aa identity within the mature region. Expression of NXPH1 and NXPH3 does not appear to overlap, with NXPH1 expression occurring mainly by cells that resemble inhibitory interneurons (2 - 4). Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 5, 6). Genetic deletion of NXPH1 and/or NXPH3 produces no anatomical effect, although mice lacking NXPH3 show specific defects in motor coordination (3, 4). Of the other known neurexophilins, NXPH2 is not expressed in rodents, and NXPH4 does not bind alpha -neurexins (1, 5).

References