Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF

Catalog #: 3098-NG
1 Citation Datasheet / COA / SDS

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3098-NG-050

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Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized at 3 μg/mL on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived rat Nogo-A protein

Rat Nogo-A (Met1-Val172) Accession # Q9JK11	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9JK11

N-terminal Sequence
Analysis

Met1

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

66-76 kDa, reducing conditions

Product Datasheets

3098-NG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3098-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Nogo-A

Nogo, so named as a “No-Go” for neurite outgrowth, is a member of the reticulon family of transmembrane proteins, and is also called reticulon 4 (gene name RTN4) (1-4). Reticulons lack N-terminal signal sequences, share a conserved ~200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER‑retention motif, and show a punctate intracellular distribution within the endoplasmic reticulum (ER) that is reminescent of a reticulum (1-3). The N-terminus of intracellular (ER) Nogo-A appears to face the cytoplasm (1-3). However, minor amounts of Nogo-A and Nogo-B are found in the plasma membrane with extracellular N-termini (4-6). Full length rat Nogo-A is a 1163 aa protein with a long (~989 aa) N-terminus that includes bioactive regions (aa 59-172 and 544-725), a transmembrane segment, a connecting loop that contains the bioactive Nogo-66 region, a second transmembrane segment, and a short C-terminus (3). The four Nogo isoforms share the Nogo66 segment, Nogo-A and Nogo-B share aa 1-172, and only Nogo-A contains aa 544-725 (1-3). Rat Nogo-A shares 78% and 91% aa sequence identity with human and mouse Nogo-A, respectively. Rat and human Nogo-A/B also share 78% aa sequence identity within aa 1-172 and 98% within the Nogo-66 loop region. Nogo-A is mainly expressed in oligodendrocytes of the central nervous system, but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages and myoblasts (1-8). Nogo-B is mainly expressed in vascular endothelium and smooth muscle throughout the body (1, 4, 6, 9). The Nogo66 region binds the GPI-linked Nogo receptor/p75 complex on axons, inducing growth cone collapse (5, 7, 10, 11). Either aa 59-172 or 544-725 segments can block neurite outgrowth and fibroblast spreading (5, 6). Nogo-A/B aa 1-172 is also reported to regulate vascular remodeling through binding the Nogo-B receptor (NgBR/NUS1) on vascular cells, and to inhibit neuronal differentiation and promote glial formation from neural progenitors (4, 5, 8, 9, 12).

References

Oertle, T. and M.E. Schwab (2003) Trends Cell Biol. 13:187.
GrandPre, T. et al. (2000) Nature 403:439.
Chen, M.S. et al. (2000) Nature 403:434.
Acevedo, L. et al. (2004) Nat. Med. 10:382.
Oertle, T. et al. (2003) J. Neurosci. 23:5393.
Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
Wang, X. et al. (2002) J. Neurosci. 22:5505.
Schwab, M.E. (2010) Nat. Rev. Neurosci. 11:799.
Miao, R.Q. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10997.
Fournier, A.E. et al. (2001) Nature 409:341.
Wang, K.C. et al. (2002) Nature 420:74.
Guo, Y. et al. (2009) Neurosci. Lett. 458:132.

Long Name

Reticulon 4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Alternate Names

ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

Related Research Areas

Citation for Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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