Recombinant Rat Nogo-A Fc Chimera Protein, CF

Catalog #: 2445-NG
2 Citations Datasheet / COA / SDS

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2445-NG-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Nogo-A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 300 ng on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived rat Nogo-A protein

Rat Nogo-A (Thr544 - Phe725) Accession # Q9JK11	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q9JK11

N-terminal Sequence
Analysis

Thr544

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46.1 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Product Datasheets

2445-NG

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2445-NG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Nogo-A

Rat Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved approximately 200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1, 2). Nogo-A in rat exists in four isoforms (3 - 5). The full length rat Nogo-A is 1163 aa in length and contains a 989 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa transmembrane connecting loop (SWISSPROT defines this region as being 94 aa in length while the original cloning papers identified it as being 66 aa in length). This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (6 - 8). Two other areas in the N-terminus have also been discovered to have bioactivity (6, 9, 10). The amino acid segment 59 - 172 is reported to block fibroblast spreading, while the aa segment 544 - 725 blocks neurite outgrowth and block fibroblast spreading (6, 10). The exact topology of Nogo-A is unclear. With two transmembrane segments, the N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (11, 12). Alternatively, the loop region and N-terminus may be on the same side of the membrane (6). Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (10, 13, 14). Rat Nogo-A is 78% aa identical to human Nogo-A overall, with 98% aa identical in the loop region and 81% aa identity in the aa 544 - 725 segment.

References

Oertle, T. et al. (2003) FASEB J. 17:1238.
GrandPre, T. et al. (2000) Nature 403:439.
Chen, M.S. et al. (2000) Nature 403:434.
Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
Ito, T. and S.M. Schwartz (1999) GenBank Accession # Q9JK11.
Oertle, T. et al. (2003) J. Neurosci. 23:5393.
Fournier, A.E. et al. (2001) Nature 409:341.
Wang, K.C. et al. (2002) Nature 420:74.
Prinjha, R. et al. (2000) Nature 403:383.
Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
Wang, X. et al. (2002) J. Neurosci. 22:5505.
Acevedo, L. et al. (2004) Nat. Med. 10:382.

Long Name

Reticulon 4A

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Alternate Names

ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

Related Research Areas

Citations for Recombinant Rat Nogo-A Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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