Recombinant Viral Dengue Virus 2 NS1 Protein, CF Summary
Product Specifications
Asp776-Ala1127, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9439-DG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Dengue Virus 2 NS1
Dengue virus type2 non-structural protein 1 (DENV2 NS1) is an approximately 50 kDa viral glycoprotein (1). Dengue viruses are mosquito-borne flaviviruses that exist in nature as four distinct serotypes (DENV1-4) (2). Dengue virus NS1 can be found associated with cell membranes, within cells and at the cell surface, or as secreted extracellular species. The secreted form of NS1 is known to be hexameric (1). Within cells, NS1 plays a significant role in virus replication and has been shown to co-localize with dsRNA and other components of viral replication complexes in viral-induced vesicle packets (1,3). Cell surface-associated and secreted NS1 are profoundly immunogenic, and both the protein itself and the antibodies it elicits have been shown to contribute to either protection or pathogenesis and innate immune evasion (1, 3). Mature DENV2 NS1 contains 352 amino acids (aa) and consists of 2 conserved N-glycosylation sites and 12 invariant cysteine residues that form six intramolecular disulfide bonds (3).Mature viral DENV2 NS1 shares 73%, 80%, and 69% aa sequence identity with DENV1, DENV3, and DENV4 NS1, respectively.
- Muller, D. et al. (2012) J. Gen. Virol. 93:771.
- VanBlargan, L.A. et al. (2013) PLOS Pathog. 9:e1003761.
- Flamand, M. et al. (1999) J. Virol. 73:6104.
Citations for Recombinant Viral Dengue Virus 2 NS1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Reduction in Anti-Dengue Virus IgG Antibody Levels with the Use of a Larvicide for Vector Control in Rural Lao People's Democratic Republic
Authors: P Lamaningao, S Kanda, T Shimono, M Kuroda, S Inthavongs, T Xaypangna, T Nishiyama
Tropical medicine and infectious disease, 2022-12-27;8(1):.
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes
Authors: PR Abraham, B R, PK N, A Kumar
Scientific Reports, 2021-12-08;11(1):23699.
Species: N/A
Sample Types: Reference Standard
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