SCOTfluor lactic acid probe 510
Tocris Bioscience | Catalog # 7448
Key Product Details
Description
Wavelength
Product Description
SCOTfluor lactic acid probe 510 is a red fluorescent probe for imaging lactic acid metabolism in vivo. Excitation and emission maxima (λ) are 485 and 605 nm, respectively. Suitable for use with flow cytometry and super-resolution microscopy.Scientific Data Images for SCOTfluor lactic acid probe 510
Application of SCOTfluor lactic acid probe 510 in HeLa cells.
TIRF microscopy image (left) of HeLa cells after incubation with SCOTfluor 510 lactate (100 μM). Multi-tracking of fluorescent particles corresponding to SCOTfluor 510 lactate labelling (right). Scale: 5 μm. Image kindly provided by Prof. Marc Vendrell, acquired by Dr Mathew Horrocks, School of Chemistry, University of Edinburgh.Licensing Information
Sold under license from the University of EdinburghOptical Data
| Emission Color | Orange |
| λabs | 485 nm |
| λem | 605 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Super Resolution Microscopy (SRM), Flow Cytometry, in vivo |
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Product Specifications for SCOTfluor lactic acid probe 510
Molecular Weight
Formula
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Purity
Chemical Name
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PubChem ID
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.31 | 10 |
Preparing Stock Solutions for SCOTfluor lactic acid probe 510
The following data is based on the product molecular weight 331.15.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.20 mL | 150.99 mL | 301.98 mL |
| 0.5 mM | 6.04 mL | 30.20 mL | 60.40 mL |
| 1 mM | 3.02 mL | 15.10 mL | 30.20 mL |
| 5 mM | 0.60 mL | 3.02 mL | 6.04 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
- Monsorno Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice. Nat.Commun. 2023 PMID: 37717033
- Benson SCOTfluors: Small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem Int.Ed.Engl. 2019 PMID: 30924239
Product Documents for SCOTfluor lactic acid probe 510
Product Specific Notices for SCOTfluor lactic acid probe 510
For research use only
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Species: HumanAssay Type: In VitroCell Line/Tissue: 293TVerified Customer | Posted 03/07/2024Primary cultured cells were pre-treated for 2 h with either control or with low glucose-containing medium. The SCOTfluor lactate was diluted in control or low glucose-medium to a concentration of 0.1 mM and applied onto cells for 1 h. Imaging was performed using a stage incubator and flow-cyto. Treament showed that lactate upregulated
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