Sheep IgG Alexa Fluor® 700‑conjugated Control Antibody

Catalog #: IC016N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC016N-100UG

Detection of Sheep IgG Isotype Control by Flow Cytometry

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Sheep IgG Alexa Fluor® 700‑conjugated Control Antibody Summary

Specificity

Serum was obtained from naive (non-immunized) sheep.

Source

Polyclonal Sheep IgG

Purification

Protein A or G purified

Immunogen

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control

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Detection of Sheep IgG Control by Flow Cytometry Human peripheral blood monocytes were stained with Sheep Anti-Human IRF5 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC4508N, filled histogram) or Sheep IgG Alexa Fluor® 700-conjugated Isotype Control Antibody (Catalog # IC016N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG