Sheep IgG APC-conjugated Antibody

Name: Sheep IgG APC-conjugated Antibody
Brand: R&D Systems
SKU: IC016A
Rating: 5 (1 reviews)

Catalog #: IC016A
2 Citations 1 Review Datasheet / COA / SDS

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IC016A

Detection of Sheep IgG Isotype Control by Flow Cytometry

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sheep IgG APC-conjugated Antibody Summary

Specificity

Serum was obtained from naive (non-immunized) sheep.

Source

Polyclonal Sheep IgG

Purification

Protein A or G purified

Immunogen

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control

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Detection of Sheep IgG Control by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or Sheep IgG APC-conjugated Isotype Control Antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Sheep IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Conditionally pathogenic genetic variants of a hematopoietic disease-suppressing enhancer
Authors: AA Soukup, DR Matson, P Liu, KD Johnson, EH Bresnick
Science Advances, 2021-12-10;7(50):eabk3521.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.
Authors: Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O
Eur J Immunol, 2013-07-04;43(8):2138-50.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control