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Sheep IgG PE-conjugated Antibody

Catalog #: IC016P 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC016P
Detection of Sheep IgG Isotype Control by Flow Cytometry
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Sheep IgG PE-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) sheep.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Sheep IgG Isotype Control by Flow Cytometry View Larger

Detection of Sheep IgG Control by Flow Cytometry Human red blood cells were stained with Sheep Anti-Human ICAM-4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7179P, filled histogram) or Sheep IgG PE-conjugated Isotype Control Antibody (Catalog # IC016P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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FAQs

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Sheep IgG PE-conjugated Antibody
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes,bone marrow Species: Mouse

Isotype control