Simple Plex Cytokine Release Panel 2
Simple Plex Cytokine Release Panel 2 Summary
The Simple Plex Human Multianalyte Assay is for the detection of human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen in plasma/serum samples.| Sample Type & Volume Required Per Well | Plasma/Serum (25 uL) |
| Sensitivity | IL-1b 0.064 pg/mL; IL-6 3rd gen 0.11 pg/mL; IL-8 0.08 pg/mL; TNF-a 2nd gen 0.3 pg/mL |
| Assay Range | IL-1b 0.4 - 1,530 pg/mL; IL-6 3rd gen 0.28 - 2,652 pg/mL; IL-8 0.19 - 1,804 pg/mL; TNF-a 2nd gen 0.3 - 1,160 |
Product Specifications
| Reactivity | Human |
| Specificity | Natural and recombinant human IL-1b, IL-6 3rd gen, IL-18, and TNF-a 2nd gen. |
| Cross-reactivity | <0.5% cross-reactivity observed with available related molecules. <50% cross-species reactivity observed with species tested. |
| Interference | No significant interference observed with available related molecules. |
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent
| Intra-Assay Precision | Inter-Assay Precision | ||||
|---|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 | |
| IL-1b | n | 16 | 16 | 16 | 16 |
| Mean (pg/mL) | 8.66 | 446 | 8.42 | 410 | |
| Standard Deviation | 0.427 | 16.5 | 0.342 | 23.3 | |
| CV (%) | 4.9 | 3.7 | 4.0 | 5.7 | |
| IL-6 3rd gen | n | 16 | 16 | 40 | 40 |
| Mean (pg/mL) | 23.1 | 876 | 23.6 | 1008 | |
| Standard Deviation | 0.899 | 72.1 | 1.82 | 77.7 | |
| CV (%) | 3.9 | 8.2 | 7.7 | 7.7 | |
| IL-8 | n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 28.7 | 1362 | 27.3 | 1463 | |
| Standard Deviation | 1.41 | 89.8 | 1.41 | 125 | |
| CV (%) | 4.9 | 6.6 | 5.2 | 8.6 | |
| TNF-a 2nd gen | n | 16 | 16 | 36 | 35 |
| Mean (pg/mL) | 6.99 | 383 | 7.81 | 408 | |
| Standard Deviation | 0.345 | 20.6 | 0.662 | 36.9 | |
| CV (%) | 4.9 | 5.4 | 8.5 | 9.0 | |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % | |
|---|---|---|---|
| IL-1b | EDTA Plasma (n=4) | 106 | 97-120 |
| Heparin Plasma (n=4) | 99 | 92-112 | |
| Serum (n=4) | 105 | 96-112 | |
| IL-6 3rd gen | EDTA Plasma (n=4) | 92 | 86-102 |
| Heparin Plasma (n=4) | 87 | 85-90 | |
| Serum (n=4) | 94 | 90-99 | |
| IL-8 | EDTA Plasma (n=5) | 89 | 75-102 |
| Heparin Plasma (n=5) | 95 | 88-102 | |
| Serum (n=3) | 92 | 88-97 | |
| TNF alpha 2nd Gen | EDTA Plasma (n=4) | 97 | 89-105 |
| Heparin Plasma (n=4) | 89 | 77-96 | |
| Serum (n=4) | 99 | 92-108 |
Specifications
FAQs
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