Simple Plex Discovery Human TfR Assay New

Catalog #: SPCKB-PS-011827 Datasheet / COA / SDS

Simple Plex Human TfR assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer

Catalog #	Availability	Size / Price
SPCKB-PS-011827	Contact us for Delivery Time.	1 Kit (1 Analyte x 72 Samples) / Please Inquire
SPCKA-PS-011827	Contact us for Delivery Time.	1 Kit (1 Analyte x 16 Samples) / Please Inquire
SPCKC-PS-011827	Contact us for Delivery Time.	1 Kit (1 Analyte x 32 Samples) / Please Inquire

Simple Plex Discovery Human TfR Ella Assay

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All TfR (Transferrin R) Simple Plex Assays

Product Details

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Discovery Human TfR Assay Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

11 pg/mL

Assay Range

65.5 - 100,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human TfR.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex Discovery assay is for the detection of human Transferrin Receptor (TfR) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	29
Mean (pg/mL)	603	33392	596	30499
Standard Deviation	29.1	2198	65.3	2059
CV%	4.8	6.6	10.9	6.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	102	102-103

Linearity

Samples containing and/or spiked with high concentrations of recombinant human TfR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Simple Plex Discovery Human TfR Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TfR (Transferrin R)

Transferrin receptors are transmembrane, homodimeric proteins that play a role in iron transport, uptake, and storage. TfR is an essential receptor, found on almost all cell types, regulated by intracellular iron concentration, and has a high affiinity for transferrin. Soluble TfR (sTfR) arises from the proteolysis of TfR into monomers that can be measured in plasma and serum. TfR2 has limited tissue distribution (hepatocytes and enterocytes), is regulated by the cell cycle, and binds transferrin poorly.

Long Name:

Transferrin Receptor

Entrez Gene IDs:

7037 (Human); 22042 (Mouse); 64678 (Rat)

Alternate Names:

CD71 antigen; CD71; CD71TRFR; p90; T9; TfR (Transferrin R); TfR (TransferrinR); TfR; TFR1; TFRC; TR; transferrin receptor (p90, CD71); transferrin receptor protein 1; TRFR