Simple Plex Human CD21 Cartridge

Catalog #: SPCKB-PS-000451 Datasheet / COA / SDS

Simple Plex Human CD21 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000451
SPCKA-PS-000451
SPCKC-PS-000451

Control Products Available

898282: Simple Plex Control for Human CD21

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All CD21 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CD21 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL), Cerebrospinal Fluid (13 uL)

Sensitivity

44.3 pg/mL

Assay Range

91.8 - 140,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human CD21.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CD21 in cerebrospinal fluid (CSF),serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (pg/mL)	925	41601	883	39241
Standard Deviation	70.6	1969	44.7	1895
CV%	7.6	4.7	5.1	4.8

Recovery

Recovery was not necessary as CD21 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human CD21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human CD21 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD21

CD21, also known as complement receptor 2 (CR2), is a 145 kDa transmembrane protein that contains 15 tandem SCR/SUSHI repeats. CD21 is primarily expressed on B cells, follicular dendritic cells, and T cells. It binds the complement component fragments iC3b, C3d, and C3d,g and forms complexes with CD19 and CD35. CD21 is required for germinal center development, antibody production, and B cell tolerance. It also functions as a cellular uptake receptor for Epstein-Barr virus.

Entrez Gene IDs:

1380 (Human); 12902 (Mouse); 289395 (Rat)

Alternate Names:

C3DR; C3DRSLEB9; CD21 antigen; CD21; Complement C3d receptor; complement component (3d/Epstein Barr virus) receptor 2; complement receptor type 2; CR2; EBV receptor; Epstein-Barr virus receptor