Simple Plex Human IL-12 p70 Cartridge

Catalog #: SPCKB-PS-000498 Datasheet / COA / SDS

Cartridges below are for plasma/serum (PS-000498) cartridges. Stocked cartridges are indicated with ST01 and available to ship in 3-4 days. Simple Plex Human IL-12 p70 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000498
SPCKA-PS-000498
SPCKC-PS-000498

Control Products Available

894957: Simple Plex Control for Human IL-12 p70

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IL-12 p70 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL)

Sensitivity

0.39 pg/mL

Assay Range

0.6 - 5,890 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Specificity

Natural and recombinant human IL-12p70.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-12p70 in cell culture supernatant (CCS), serum, and EDTA plasma.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (pg/mL)	36.2	1657	33.2	1587
Standard Deviation	1.25	90	1.95	137
CV%	3.4	5.4	5.9	8.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=6)	87	73-117
Serum (n=6)	81	73-90

Linearity

Samples containing and/or spiked with high concentrations of human IL-12p70 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Human IL-12 p70 Simple Plex Assay Standard Curve

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Human IL-12 p70 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:

Interleukin 12 p70

Entrez Gene IDs:

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names:

CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35