Simple Plex Human IL-18 BPa Cartridge

Catalog #: SPCKB-PS-000502 Datasheet / COA / SDS

Simple Plex Human IL-18 BPa assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000502
SPCKA-PS-000502
SPCKC-PS-000502

Control Products Available

898096: Simple Plex Control for Human IL-18 BPa

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human IL-18 BPa Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bronchoalveolar Lavage Fluid (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.881 pg/mL

Assay Range

2.8 - 4,336 pg/mL (Bronchoalveolar Lavage Fluid, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IL-18 BPa.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-18 BPa in serum, plasma (EDTA/Heparin), and bronchoalveolar lavage fluid (BALF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	23	25
Mean (pg/mL)	44.9	2521	43.7	2248
Standard Deviation	2.3	232	3.22	278
CV%	5.1	9.2	7.4	12.4

Recovery

Recovery was not necessary as IL-18 BPa shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human IL-18 BPa were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

Human IL-18 BPa Simple Plex Assay Standard Curve

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Human IL-18 BPa Ella Assay Standard Curve

N/A

Human IL-18 BPa Simple Plex Assay Linearity

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Human IL-18 BPa Ella Assay Linearity

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18 BPa

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

Long Name:

Interleukin 18 Binding Protein Isoform a

Entrez Gene IDs:

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Alternate Names:

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa